贞芪酪蛋白肽药物血清诱导Bel-7402细胞分化的体外实验研究

张延英1; 朱建坤1; 吴建军1; 李广远1; 陈玉霞2; 徐琳3; 张生萍4

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贞芪酪蛋白肽药物血清诱导Bel-7402细胞分化的体外实验研究

更新时间：2024-01-04

- 贞芪酪蛋白肽药物血清诱导Bel-7402细胞分化的体外实验研究
- Experimental Study on Differentiation of Human Hepatocarcinoma Cells Induced by ligustri-astragali casein co-peptid Drug Serum in Vitro
- 中国实验方剂学杂志 2008年14卷第9期页码：47-50
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- 纸质出版日期：2008
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张延英1, 朱建坤1, 吴建军1, 等. 贞芪酪蛋白肽药物血清诱导Bel-7402细胞分化的体外实验研究[J]. 中国实验方剂学杂志, 2008,14(9):47-50.

Experimental Study on Differentiation of Human Hepatocarcinoma Cells Induced by ligustri-astragali casein co-peptid Drug Serum in Vitro[J]. Chinese journal of experimental traditional medical formulae, 2008, 14(9): 47-50.
张延英1, 朱建坤1, 吴建军1, 等. 贞芪酪蛋白肽药物血清诱导Bel-7402细胞分化的体外实验研究[J]. 中国实验方剂学杂志, 2008,14(9):47-50. DOI：

Experimental Study on Differentiation of Human Hepatocarcinoma Cells Induced by ligustri-astragali casein co-peptid Drug Serum in Vitro[J]. Chinese journal of experimental traditional medical formulae, 2008, 14(9): 47-50. DOI：

摘要

目的:探讨贞芪酪蛋白复合肽对Bel-7402人肝癌细胞分化的诱导作用

及对其端粒酶活性的影响。方法:贞芪酪蛋白复合肽给正常大鼠灌胃

采血制备药物血清。以正常鼠血清为阴性对照

维甲酸(RA)为阳性对照

将药物血清温育Bel-7402人肝癌细胞。噻唑蓝(MTT)比色法测定细胞增殖

图像分析系统测定细胞核质比

放射免疫法测定甲胎蛋白(AFP)含量

动态比色法测定γ-谷氨酰转肽酶(-γGT)和碱性磷酸酶(ALP)活性

多聚酶链反应—酶联免疫吸附法(PCR-ELISA法)检测细胞端粒酶活性。结果:贞芪酪蛋白复合肽药物血清可抑制人肝癌细胞的增殖

显著降低细胞核质比

抑制细胞分泌AFP

降低细胞-γGT活性

升高ALP活性

并有显著抑制人肝癌细胞端粒酶活性的作用。结论:贞芪酪蛋白复合肽具有诱导人肝癌细胞分化的作用

其主要机理可能是抑制了Bel-7402细胞端粒酶活性。

Abstract

Objective: To study the effect of ligustri-astragali casein co-peptid(LACP)drug serum in inducing differentiation of Bel-7402 human hepatocarcinoma cell(HHC) and its influence of the telomerase activity.Methods: Rats were fed with LACP decoction to prepare the drug-serum of LACP

and the drug-serum was used for Bel-7402 HHC culture.Serum of normal rat

was taken as negative control and that of retinoic acid(RA) as the positive control.Cell proliferation was detected by MTT colorimetric assay

nucleocytoplasm ratio by image assay

α-fetoprotein(AFP) by RIA

alkaline phosphatase(ALP) activity and γ-glutamyl transpeptidase(γ-GT) by dynamic colorimetric assay and telomerase activity of Bel-7402 cell by PCR-ELISA.Results: Drug-serum of FACP could inhibit the proliferation of Bel-7402 cell

markedly reduce the nucleocytoplasm ratio and the secretion of AFP

decrease γ-GT activity

increase ALP activity and suppress the telomerase activity of Bel-7402 cell.Conclusion: LACP can induce the differentiation of Bel-7402 HHC

the main mechanism maybe throngh the suppression of telomerase activity.

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