三叶青提取物对肺癌A549细胞的体外抑制作用

程伟; 陆曙梅

您当前的位置：

首页 >

文章列表页 >

三叶青提取物对肺癌A549细胞的体外抑制作用

更新时间：2024-01-04

- 三叶青提取物对肺癌A549细胞的体外抑制作用
- Depressant Efects of the Extract of Radix Tetrastigma Hemsleyani On Lung Carcinoma Cell Line A549 in vitro
- 中国实验方剂学杂志 2007年13卷第10期页码：53-56
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  中图分类号：
- 纸质出版日期：2007
- 稿件说明：
移动端阅览
程伟, 陆曙梅. 三叶青提取物对肺癌A549细胞的体外抑制作用[J]. 中国实验方剂学杂志, 2007,13(10):53-56.

CHENG Wei, LU Shu-mei. Depressant Efects of the Extract of Radix Tetrastigma Hemsleyani On Lung Carcinoma Cell Line A549 in vitro[J]. Chinese journal of experimental traditional medical formulae, 2007, 13(10): 53-56.
程伟, 陆曙梅. 三叶青提取物对肺癌A549细胞的体外抑制作用[J]. 中国实验方剂学杂志, 2007,13(10):53-56. DOI：

CHENG Wei, LU Shu-mei. Depressant Efects of the Extract of Radix Tetrastigma Hemsleyani On Lung Carcinoma Cell Line A549 in vitro[J]. Chinese journal of experimental traditional medical formulae, 2007, 13(10): 53-56. DOI：

摘要

目的:探讨三叶青提取物对肺癌细胞A549的体外抑制作用。方法:采用体外细胞培养技术

用MTT法观察三叶青提取物对肺癌细胞A549体外抑制作用的时效、量效关系;琼脂糖凝胶电泳检测细胞凋亡;RT-PCR法检测细胞凋亡相关基因BcL-2的表达。结果:三叶青提取物以1×10-3g·L-1的浓度与A549细胞共培养48h

其细胞活性有显著降低;不同浓度的三叶青提取物和A549细胞共同培养

药物浓度为1×10-2、1×10-3、1×10-4g·L-1的三叶青提取物对A549细胞活性有显著性抑制作用。琼脂糖凝胶电泳有明显的梯状条带;不同浓度三叶青提取物作用于A549细胞后

细胞BcL-2基因的表达随浓度的增加而降低。结论:三叶青提取物对A549细胞具有明显的体外抑制作用

其抑制作用与药物的浓度和作用时间呈明显正相关。

Abstract

Objective:To study depressant efects of the extract of Radix Tetrastigma Hemsleyani on lung carcinoma cell line A549 in vitro.Methods:The time-effect and quality-effect of the extract of Radix Tetrastigma Hemsleyani on lung carcinoma cell line A549 were observed by cell culturein vitro using the method of MTT.Agarose gel electrophoresis was used to detect the apoptosis.RT-PCR were used to detect the expression of BcL2 gene

which was related to apoptosis.Results:48 hours after co-culturing A549 and Radix Tetrastigma Hemsleyani with a concentration of 1×10-3 g·L-1.the cell activity decreased significantly compared to control.When culturing A549 and the extract with different concentrations

it had a significant depressant effect on cell activity with concentrations of 1×10-2

1×10-3

1×10-4 g·L-1.There was an obvious apoptotic ladder in agarose gel electrophoresis.Culturing A549 and the extract with different concentrations

the expression of BcL-2 gene was low following the density raise of Radix Tetrastigma Hemsleyani.Conclusions:the extract of Radix Tetrastigma Hemsleyani has a significant depressant effect on lung carcinoma cell line A549

which positively relates with its concentration and efect-time.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

暂无数据