Objective:To study the isolation of the active in vitro cell membranes of pig intestines and adsorption of astragaloside IV and ferulic acid in Buyang Huanwu Decoction(BYHWD).Methods:The sucrose density gradient ultracentrifugection at different speeds was used to isolate pig intestinal membranes and determine it's total protein content.Normal column chromatography was used to isolate the astragaloside IV and ferulic acid

the content of which was determined by HPLC analysis.Results:The content of total protein in the cell membrane is 8.0%±0.45%

The peak time for astragaloside IV is 1 h

while the concentration is 123.6 μg·mL-1 and the transferring ratio is 94.46%;The peak time for ferulic acid is 9 h

while the concentration is 8.769 μg·mL-1 and the transferring ratio is 86.78%.Conclusions:There are significant difference in adsorbability and transporting capability between astragaloside IV and ferulic acid under cell membrane chromatography.The cell membrane could be used to separate the ingredients of the compound formulate of traditional Chinese medicine.