Objective:To study the qualitative and quantitative methods of Liangge Pills.Methods: TLC was used to identify Radix Scutellariae

Fructus Gardenia;HPLC was used to determine rheinic acid

emodin and chrysophanol in the preparation.The samples were separated on an Alltima ODS C18(4.6 mm × 250 mm

5 μm) column with methanol-acetonitril-0.1% phosphoric(40:40:20) as the mobile phase.The detection wavelength was set at 254 nm and the flow rate was 1.0 mL·min-1.Results: Radix Scutellariae

Fructus Gardeniae could be indentified by TLC.The calibration curve was linear in the range of((0.055 86)～(0.558 6)) μg

r=(0.999 6)

for rheinic acid

the average recovery was 100.94%

RSD=1.25%(n=6);The calibration curve was linear in the range of((0.079 8)～(0.798 0)) μg

r=(0.999 7)

for emodin

the average recovery was 102.49%

RSD=1.42%(n=6);The calibration curve was linear in the range of((0.063 84)～(0.638 4)) μg

r=(0.999 8)

for chrysophanol

the average recovery was 103.24%

RSD=0.85%(n=6).Conclusion: The methods are simple

reliable

and it can be used for quality control of Liangge Pills.