Objective:To develop a TLC method for Caulis Sinomenii indentification and a HPLC method for Sinomenine determination in the anti gout granules.Methods:Caulis Sinomenii was identified on a silicagel thin layer plate developed with methylbenzene∶acetidin∶methanol∶ammonia water(2∶4∶2∶1)and with sinomenine as the chemical reference substance

improved.Dragendorff reagent was used for visualization.The separation of Sinomenium was carried out on column Extend C18 4.6 mm×150 mm

5 μm.The mobile phase was the combination of methanol and the solution of 0.1% phosphoric acid(38∶62)

and the detection wareleng the was set at 262 nm.Result:Sinomenine spots on TLC plates was separated detection warelength well.The linear range is between 0.414 and 2.484 μg(r=(0.999 8)) and the recovery is 97.6%(RSD=0.32%).Conclusions: The methods are sensitive

simple

accurate and can be used for the control of anti gout granules.