建立高效液相指纹图谱分析无机离子对丹参不定根有效成分影响

王学勇; 崔光红; 何希荣; 黄璐琦

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建立高效液相指纹图谱分析无机离子对丹参不定根有效成分影响

更新时间：2024-09-23

- 建立高效液相指纹图谱分析无机离子对丹参不定根有效成分影响
- The Fingerprint for Adventitious Root of Developed by High-performance Liquid Chromatography and its Active Component Primary Analysis
- 中国实验方剂学杂志 2007年13卷第1期页码：1-4
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- 纸质出版日期：2007
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王学勇, 崔光红, 何希荣, 等. 建立高效液相指纹图谱分析无机离子对丹参不定根有效成分影响[J]. 中国实验方剂学杂志, 2007,13(1):1-4.

WANG Xue-yong, CUI Guang-hong, HE Xi-rong, et al. The Fingerprint for Adventitious Root of Developed by High-performance Liquid Chromatography and its Active Component Primary Analysis[J]. Chinese journal of experimental traditional medical formulae, 2007, 13(1): 1-4.
王学勇, 崔光红, 何希荣, 等. 建立高效液相指纹图谱分析无机离子对丹参不定根有效成分影响[J]. 中国实验方剂学杂志, 2007,13(1):1-4. DOI：

WANG Xue-yong, CUI Guang-hong, HE Xi-rong, et al. The Fingerprint for Adventitious Root of Developed by High-performance Liquid Chromatography and its Active Component Primary Analysis[J]. Chinese journal of experimental traditional medical formulae, 2007, 13(1): 1-4. DOI：

摘要

目的:建立丹参不定根RP-HPLC指纹图谱分析方法

分析无机离子对丹参不定根影响。方法:高效液相色谱法

RP-Waters C18(150 mm×3.9 mm

5 μm)

流动相为0.5%醋酸甲醇和0.5%醋酸水梯度洗脱

流速1.0mL·min-1

检测波长:270 nm

柱温:30℃。对丹参不定根进行指纹图谱分析。结果:在此色谱条件下

3组共9份样品的不定根可检测出11个相对位置稳定的共有峰

其中确定9个共有峰作为成分变化指示峰

并确定了5个已知成分

结果显示无机金属离子能明显提高不定根中丹酚酸类成分的含量

而对丹参酮等脂溶性成分影响不显著。结论:该方法稳定可靠

重复性强

可用于评价对丹参不定根次生代谢成分变化情况

为丹参不定根的培养及进一步研究奠定基础。

Abstract

Objective:To establish RP-HPLC fingerprint for adventitious root of Radix Salviae Miltiorrhizae and primarily investigate effects of different mental ion on it.Method:The Methanol extraction of Radix Salviae Miltiorrhizae was filtered through 0.45 mm filters and separated by using a Resolve TM C18 column(Waters

150 mm×3.9 mm

5 μm) at 30℃.The mobile phase consisted of acetonitrile and water was used as gradient elution for the separation.The flow rate was set at 1.0 mL·min-1 and detection was set at 270 nm.The fingerprint was then established as above condition for adventitious root of Radix Salviae Miltiorrhizae Result:11 peaks in common of 9 samples of treated with 3 kind of metal ion to adventitious root were acquired and 9 of them were chosen as symbolization for the component of Radix Salviae Miltiorrhizae.Conclution:The fingerprint profile provides a good approach to value the quality and monitor the change of content of active component in adventitious root of Radix Salviae Miltiorrhizae.

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