HPLC-ELSD法对猪胆粉中猪去氧胆酸的含量测定研究

李志万1; 马强2; 袁永生3; 周玉新3

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HPLC-ELSD法对猪胆粉中猪去氧胆酸的含量测定研究

更新时间：2024-09-23

- HPLC-ELSD法对猪胆粉中猪去氧胆酸的含量测定研究
- Determination of Hyodeoxycholic Acid in Porcine Gall Powder by HPLC with Evaporative Light-Scattering Detector
- 中国实验方剂学杂志 2005年11卷第5期页码：14-16
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- 纸质出版日期：2005
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李志万1, 马强2, 袁永生3, 等. HPLC-ELSD法对猪胆粉中猪去氧胆酸的含量测定研究[J]. 中国实验方剂学杂志, 2005,11(5):14-16.

LI Zhi-wan1, MA Qiang2, YUAN Yong-sheng3, et al. Determination of Hyodeoxycholic Acid in Porcine Gall Powder by HPLC with Evaporative Light-Scattering Detector[J]. Chinese journal of experimental traditional medical formulae, 2005, 11(5): 14-16.
李志万1, 马强2, 袁永生3, 等. HPLC-ELSD法对猪胆粉中猪去氧胆酸的含量测定研究[J]. 中国实验方剂学杂志, 2005,11(5):14-16. DOI：

LI Zhi-wan1, MA Qiang2, YUAN Yong-sheng3, et al. Determination of Hyodeoxycholic Acid in Porcine Gall Powder by HPLC with Evaporative Light-Scattering Detector[J]. Chinese journal of experimental traditional medical formulae, 2005, 11(5): 14-16. DOI：

摘要

目的:建立猪胆粉及其制品中猪去氧胆酸的含量测定方法。方法:以Kromasil C18(4.6mm×250mm

5μm)色谱柱为固定相

柱温25℃

以乙腈-0.1%乙酸水溶液(50∶50)为流动相

流速1.0mL·min-1；蒸发光散射检测器检测

漂移管温度40℃

以氮气为载气

压力2.0bar。结果:猪去氧胆酸在5.4μg～27.0μg范围内线性关系良好

r=0.9994

加样回收率为97.0%

RSD=2.7%(n=5)。结论:该方法简便、准确、重复性好。

Abstract

Objective:To develop a method for the determination of hyodeoxycholic acid in Porcine Gall Powder.Method:Chromatography was performed on a 5 μm Kromasil C18(4.6 mm×250 mm)at 25℃.The mobile phase was composed of acetonitrile-0.1% acetic acid solution (50∶50)with a flow rate of 1.0mL·min-1.A Sedex 55 evaporative light-scattering detector was used with tube temperature of 40℃ and gas pressure of 2.0 bar.Result:A good relationship between peak area and amount of injection Was noted at 5.4 μg～27.0 μg.Average recovery of hyodeoxycholic acid was 97.0%and the RSD was 2.7%.Conclusion:It was proved that the method was reliable

simple and accurate.

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