Objective:To create a quantitative analysis method of RP-HPLC to determine the serum concentration of berberine from QLS in rabbits.Methods:The conditions of chromatography:stationary phase:Platinum EPS column (C8 100A 3μ

150mm×4.6mm)

mobile phase:acetonitrile～1/12N phosphate buffer solution(70:30

v/v)

flow rate:0.8ml·min-1

detection wavelength was 345 nm

temperature of column was 40℃

external standard method was quantitative analysis method.Results:The linear relationship of serum concentration of berberine was shown well in the range of 0.72μg·L-1～578.98μg·L-1

the detectability was 0.36μg·L-1

recovery ratio was(96.06±4.74)%

degree of precision of within the day and among the day were 1.16%～2.18%、0.99%～3.04% respectively.Conclusion:This method was shown with good accuracy、good sensitivity and good specificity

can provide a valuable method for serum concentration detection of berberine.