Objective: To develop an HPLC method for quantitative analysis of curcuminoids in Huangsi Yujin and Lvsi Yujin derived from Cucurmae Radix. Method: Ten batches of samples were collected respectively

and were extracted with methanol in ultrasonic bath for 60 min

the separation was performed on a YMC ODS-A C18 reversed column (4.6 mm×250 mm

5 μm)

the mobile phase consisted of acetonitrile and 0.1% formic acid in water (45:55)

the mobile phase flow rate was 1.0 mL·min-1 and the UV detector was monitored 425 nm for quantitative analysis. Result: The linear of curcumin

demethoxycurcumin

bisdemethoxycurcumin were within 3.516-450 mg·L-1 (r=0.999 8)

2.734-350 mg·L-1(r=0.999 9) and 2.422-310 mg·L-1(r=0.999 8) respectively. The average recoveries of curcumin

demethoxycurcumin

bisdemethoxycurcumin were 101.36% (RSD 2.62%

n=5)

102.19% (RSD 2.75%

n=5) and 102.86% (RSD 3.06%

n=5) respectively. Conclusion: The validated HPLC method could be used as quality control method for Cucurmae Radix. Curcuminoids were only detected in Huangsi Yujin

while not detected in Lvsi Yujin.