Objective: To investigate pharmacokinetics process of the major components of the total phenolic acids of Salvia miltiorrhiza in rats. Method: Using HPLC to measure plasma concentration at different time after ig total phenolic acids of S. miltiorrhiza was determined; DAS 3.0 software was used to calculate pharmacokinetic parameters. Result: Lithospermic acid was linear in the range of 1.0-20.0 mg·L-1.Absolute recoveries were between 97.15%-100.21%.The RSD of intra-day and inter-day was less than 10.23%.Salvianolic acid B was linear in the range of 2.0-20.0 mg·L-1.Absolute recoveries were between 94.80%-99.96%. The RSD of intra-day and inter-day was less than 10.06%. Pharmacokinetic parameters of lithospermic acid were as follows:t1/2=19.4 min

Cmax=14.9 mg·L-1

AUC=418.0 μg·L-1·min-1. Pharmacokinetic parameters of salvianolic acid B were as follows:t1/2=27.2 min

Cmax=17.1 mg·L-1

AUC=831.3 μg·L-1·min-1. Conclusion: HPLC had good selectivity

high sensitivity for the determination of plasma sample. for the pharmacokinetic studies of lithospermic acid and salvianolic acid B in total phenolic acids of S. miltiorrhiza.