NIU Xiao-hong, BAI Dong, SONG Jian-nan. Content Determination of Total Polysaccharide in the Guizhitang Decoction by Colorimetry[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(13): 66-68.
NIU Xiao-hong, BAI Dong, SONG Jian-nan. Content Determination of Total Polysaccharide in the Guizhitang Decoction by Colorimetry[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(13): 66-68. DOI: 10.11653/syfj2013130066.
Objective:To develop a method for content determination of total polysaccharide in Guizhitang decoction. Method: Glucose was used as the reference substance
and phenol-sulfuric acid was applied as coloring reagent to determine the content of total polysaccharide. Result: The calibration curve of glucose showed good linearity in the range of 19.62-117.70 mg·L-1. The average recovery rate was 99.28%
and RSD was 2.5% (n=6). Conclusion: The determination method is simple
sensitive and accurate
which can be used for evaluating the content of total polysaccharide in Guizhitang decoction.