款冬花的高效毛细管电泳指纹图谱

王国艳; 郝增燕; 胡明勋; 张红芬; 陈安家

doi:10.11653/syfj2013130097

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款冬花的高效毛细管电泳指纹图谱

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- 款冬花的高效毛细管电泳指纹图谱
- Fingerprints of Flos Farfarae by High Performance Capillary Electrophoresis
- 中国实验方剂学杂志 2013年19卷第13期页码：97-100
- 作者机构：
- 作者简介：
- 基金信息：
  
  山西省归国人员基金项目(2010-53)
- DOI：10.11653/syfj2013130097
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
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王国艳, 郝增燕, 胡明勋, 等. 款冬花的高效毛细管电泳指纹图谱[J]. 中国实验方剂学杂志, 2013,19(13):97-100.

WANG Guo-yan, HAO Zeng-yan, HU Ming-xun, et al. Fingerprints of Flos Farfarae by High Performance Capillary Electrophoresis[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(13): 97-100.
王国艳, 郝增燕, 胡明勋, 等. 款冬花的高效毛细管电泳指纹图谱[J]. 中国实验方剂学杂志, 2013,19(13):97-100. DOI： 10.11653/syfj2013130097.

WANG Guo-yan, HAO Zeng-yan, HU Ming-xun, et al. Fingerprints of Flos Farfarae by High Performance Capillary Electrophoresis[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(13): 97-100. DOI： 10.11653/syfj2013130097.

摘要

目的: 建立款冬花的高效毛细管电泳(HPCE)指纹图谱

为款冬花的质量控制提供依据。方法: 采用未涂层毛细管柱(75 μm×57 cm

有效长度50 cm)为分离通道

以30 mmol·L-1硼砂(含甲醇25%

pH 8.8)溶液为背景电解质

分离电压20 kV

检测波长214 nm

柱温25 ℃。结果: 测定了15批款冬花药材的高效毛细管电泳指纹图谱

确定了26个共有峰并指认了6个峰。结论: 该方法操作简单、准确可靠

为科学评价款冬花药材的质量提供依据。

Abstract

Objective:To develop high performance capillary electrophoresis (HPCE) fingerprints of Flos Farfarae and to provide the scientific basis for quality control of it. Method: Electrophoresis was performed on a 75 μm× 57 cm (effective length is 50 cm) fused silica capillary. 30 mmol·L-1 sodium borate contained 25% methanol adjusted to pH 8.8 was applied as the background electrolyte. The running voltage was 20 kV

the detection wavelength was at 214 nm and the temperature was kept at 25 ℃. Result: The HPCE fingerprint established from the 15 batches of the Flos Farfarae showed 26 characteristic common peaks

among which 6 peaks were recognized. Conclusion: The method is reliable

accurate

simple and quick. It provides a reliable method for the scientific quality evaluation of Flos Farfarae.

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