HPLC同时测定扶正固本颗粒中黄芩苷和淫羊藿苷

李建伟; 海丽娜; 聂磊; 刘娟

doi:10.11653/syfj2013130128

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HPLC同时测定扶正固本颗粒中黄芩苷和淫羊藿苷

更新时间：2024-01-04

- HPLC同时测定扶正固本颗粒中黄芩苷和淫羊藿苷
- Simultaneous Determination of Baicalin and Icariin in Fuzheng Guben Granule by HPLC
- 中国实验方剂学杂志 2013年19卷第13期页码：128-130
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- DOI：10.11653/syfj2013130128
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- 纸质出版日期：2013
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李建伟, 海丽娜, 聂磊, 等. HPLC同时测定扶正固本颗粒中黄芩苷和淫羊藿苷[J]. 中国实验方剂学杂志, 2013,19(13):128-130.

LI Jian-wei, HAI Li-na, NIE Lei, et al. Simultaneous Determination of Baicalin and Icariin in Fuzheng Guben Granule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(13): 128-130.
李建伟, 海丽娜, 聂磊, 等. HPLC同时测定扶正固本颗粒中黄芩苷和淫羊藿苷[J]. 中国实验方剂学杂志, 2013,19(13):128-130. DOI： 10.11653/syfj2013130128.

LI Jian-wei, HAI Li-na, NIE Lei, et al. Simultaneous Determination of Baicalin and Icariin in Fuzheng Guben Granule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(13): 128-130. DOI： 10.11653/syfj2013130128.

摘要

目的: 建立扶正固本颗粒中黄芩苷和淫羊藿苷的含量测定方法。方法: 采用HPLC法

以乙腈-0.2%磷酸溶液梯度洗脱

色谱柱为日旭AQ C18(4.6 mm×250 mm

5 μm)

流速1.0 mL·min-1

柱温25 ℃

检测波长270 nm。结果: 黄芩苷在0.756~10.08 μg

淫羊藿苷在0.299~39.92 μg与峰面积呈良好线性关系

Abstract

Objective:To develop a quantitative method for determining the contents of baicalin and icariin in Fuzheng Guben granule. Method: The analysis was performed with a Ultimate AQC18(4.6 mm×250 mm

5 μm) column and a mobile phase of acetonitrile-0.2% phosphoric acid solution with gradient elution. The flow rate was 1.0 mL·min-1

and column temperature was 25 ℃. The detection wavelength was set at 270 nm. Result: The calibration curve was linear within the range of 0.756-10.08 μg for baicalin(r=1.000 0) and 0.299-39.92 μg for icariin(r=0.999 6). The average recoveries of the two contents were 101.40%(RSD 1.41%)and 101.02%(RSD 1.08%) respectively. Conclusion: This method is convenient

accurate and reliable

and can be used as quality control methods for Fuzheng Guben granule.

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