Objective:To investigate the effect of Huiru Yizeng Ⅰ on hyperplasia of mammary glands in rats

and explore the mechanism. Method: Eighty rats were divided into control group

disease model group

integrated disease model group

tamoxifen group(1.57 mg·kg-1)

Rupi Sanjie group(0.50 g·kg-1)

Huiru Yizeng Ⅰ high(31.6 g·kg-1)

middle(15.80 g·kg-1)and low dose(7.9 g·kg-1)group. The model of mammary gland hyperplasia was induced by im benzoate estradiol and progesterone. The model of integrated disease was made by cliping the tail of every rat with a tong after experiencing mammary gland hyperplasia. The model rats were treated with different drugs for 4 weeks respectively. The breast diameter

body weight and serum hormone(determined by ELISA)was determine and pathomorphology change of glandular tissue in all groups was detected by microscope. Result: Huiru Yizeng Ⅰ could significantly diminish the breast diameter(P<0.01)and restore the body weight of rats(P<0.01)

decrease serum estradiol(E2) [(2.387±0.22) pmol·L-1

P<0.01]

prolactin (PRL)[(300.17±17.26) ng·L-1

P<0.05]

follicle-stimulating hormone(FSH) [(0.42±0.019) U·L-1

P<0.05)]

gonadotropin releasing hormone(GnRH) [(1.175±0.35) ng·L-1

P<0.01] and 5-hydroxy tryptamine(5-HT) [(18.179±2.053)ng·L-1

P<0.05]

increase progesterone (P) [(0.99±0.078) μg·L-1

P<0.05]

luteinizing hormone(LH) [(1.724±0.254) U·L-1

P<0.01)

and significantly lessen the amount of lobules of mammary gland

gland alveolus and secretion. Conclusion: Huiru Yizeng Ⅰ can remarkably improve the disorder of serum hormone

and decrease the occurrence of hyperplasia of mammary glands.