大孔吸附树脂纯化倒卵叶五加中紫丁香苷的工艺优选

曹爱兰; 史文静; 张俏; 杜文娟

doi:10.11653/syfj2013140062

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大孔吸附树脂纯化倒卵叶五加中紫丁香苷的工艺优选

更新时间：2024-01-04

- 大孔吸附树脂纯化倒卵叶五加中紫丁香苷的工艺优选
- Optimization of Separation and Purification Technology of Syringin in with Macroporous Absorption Resin
- 中国实验方剂学杂志 2013年19卷第14期页码：62-64
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- DOI：10.11653/syfj2013140062
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- 纸质出版日期：2013
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曹爱兰, 史文静, 张俏, 等. 大孔吸附树脂纯化倒卵叶五加中紫丁香苷的工艺优选[J]. 中国实验方剂学杂志, 2013,19(14):62-64.

CAO Ai-lan, SHI Wen-jing, ZHANG Qiao, et al. Optimization of Separation and Purification Technology of Syringin in with Macroporous Absorption Resin[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(14): 62-64.
曹爱兰, 史文静, 张俏, 等. 大孔吸附树脂纯化倒卵叶五加中紫丁香苷的工艺优选[J]. 中国实验方剂学杂志, 2013,19(14):62-64. DOI： 10.11653/syfj2013140062.

CAO Ai-lan, SHI Wen-jing, ZHANG Qiao, et al. Optimization of Separation and Purification Technology of Syringin in with Macroporous Absorption Resin[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(14): 62-64. DOI： 10.11653/syfj2013140062.

摘要

目的: 优选大孔吸附树脂纯化倒卵叶五加中紫丁香苷的工艺条件。方法: 采用HPLC测定紫丁香苷含量

通过静态吸附-洗脱试验考察大孔树脂型号

利用单因素试验考察上样量、吸附流速、洗脱机浓度及用量对纯化工艺的影响。结果: 选用XDA-1型大孔树脂

其纯化工艺条件为上样量每g树脂吸附3.5 mg紫丁香苷

吸附流速1 BV·h-1

用9 BV 40%乙醇于2 BV·h-1流速洗脱

紫丁香苷纯度由5.4%提高到23.1%。结论: XDA-1型大孔树脂可用于纯化倒卵叶五加提取液

紫丁香苷收率和纯度均达满意效果。

Abstract

Objective: To optimize separation and purification technology of syringin in Acanthopanax obouatus with macroporous absorption resin. Method: With the content of syringin as index

which was determined by HPLC

model of macroporous absorption resin was screened by static adsorption-elution test

purification conditions were investigated by single factor tests. Result: XDA-1 macroporous adsorption resin model was adopted

its purification technology was as following:the amount of sample 3.5 mg syringin with 1 g XDA-1 macroporous absorption resin

adsorption velocity 1 BV·h-1

eluted with 9 BV 40% ethanol at 2 BV·h-1.Purity of syringin was up to 28.14% from 5.47%. Conclusion: XDA-1 macroporous resin could be used to purify extract of A. obouatus

yield and purity of syringin obtained satisfactory results.

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