HPLC测定散结镇痛胶囊中龙血素A,B含量

鹿丽丽; 萧伟; 徐连明; 徐忠坤; 殷洪梅

doi:10.11653/syfj2013140105

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HPLC测定散结镇痛胶囊中龙血素A,B含量

更新时间：2024-01-04

- HPLC测定散结镇痛胶囊中龙血素A,B含量
- Determination of Loureirin A, B in Sanjie Zhentong Capsules by HPLC
- 中国实验方剂学杂志 2013年19卷第14期页码：105-108
- 作者机构：
- 作者简介：
- 基金信息：
  
  科学技术部国家重大新药创制项目(2011ZX09201-201-20)
- DOI：10.11653/syfj2013140105
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
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鹿丽丽, 萧伟, 徐连明, 等. HPLC测定散结镇痛胶囊中龙血素A,B含量[J]. 中国实验方剂学杂志, 2013,19(14):105-108.

LU Li-li, XIAO Wei, XU Lian-ming, et al. Determination of Loureirin A, B in Sanjie Zhentong Capsules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(14): 105-108.
鹿丽丽, 萧伟, 徐连明, 等. HPLC测定散结镇痛胶囊中龙血素A,B含量[J]. 中国实验方剂学杂志, 2013,19(14):105-108. DOI： 10.11653/syfj2013140105.

LU Li-li, XIAO Wei, XU Lian-ming, et al. Determination of Loureirin A, B in Sanjie Zhentong Capsules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(14): 105-108. DOI： 10.11653/syfj2013140105.

摘要

目的: 建立散结镇痛胶囊中龙血素A、龙血素B的含量测定方法。方法: 75%甲醇超声提取龙血素A、龙血素B

以Kromasil C18(4.6 mm×250 mm

5 μm)为分析柱

以乙腈-1%冰醋酸为流动相进行梯度洗脱

流速1 mL·min-1

进样量10 μL

检测波长278 nm

柱温30℃。结果: 龙血素A在526.20~4.11 ng线性关系良好(r=0.999 9)

平均回收率101.84%(RSD 0.77%

n=6);龙血素B在506.09~3.95 ng线性关系良好

r=0.999 9

平均回收率100.54%(RSD 1.46%

n=6);并具有较好的重复性和稳定性。结论: 样品处理方法合理

方法学考察符合定量要求

结果准确

可用于散结镇痛胶囊中龙血素A、龙血素B的含量测定。

Abstract

Objective: To establish an HPLC method for content determination of loureirin A

B in Sanjie Zhentong capsules. Method: Extracted loureirin A

B with ultrasonic method in the use of 75% methanol

Kromasil C18 column (4.6 mm×250 mm

5 μm) was used with a step gradient of acetonitrile-1% glacial acetic acid at a flow rate of 1.0 mL·min-1. The sample amount was 10 μL and the detection wavelength was set at 278 nm. The column temperature was maintained at 30℃. Result: Loureirin A and B had a good linear relationship during 526.20-4.11 ng (r=0.999 9) and 506.09-3.95 ng (r=0.999 9) respectively. The average recoveries were 101.84% with RSD 0.77%(n=6)

100.54% with RSD 1.46%(n=6). The method also had good reproducibility and stability. Conclusion: The sample preparation is reasonable in the quantitative method

and the accurate results can be used for content determination of loureirin A and B of Sanjie Zhentong Capsules.

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