YIN Xing-bin, LIN Long-fei, CAO Sa-li, et al. Simultaneous Determination of Polyphyllin Ⅰ,Ⅱ, Ⅵ,Ⅶ and Hyperoside in Chonglou Kegan Capsule[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(15): 58-61.
YIN Xing-bin, LIN Long-fei, CAO Sa-li, et al. Simultaneous Determination of Polyphyllin Ⅰ,Ⅱ, Ⅵ,Ⅶ and Hyperoside in Chonglou Kegan Capsule[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(15): 58-61. DOI: 10.11653/syfj2013150058.
并使用本方法测定3批重楼克感胶囊中5种化合物的含量。 方法: Dikma C18(2)色谱柱(4.6 mm×250 mm
5 μm)
乙腈-0.1%磷酸 (42:58) 为流动相
检测波长203
360 nm
流速1.0 mL·min-1
柱温30℃。 结果: 重楼皂苷Ⅰ
Ⅱ
Ⅵ
Ⅶ及金丝桃苷在0.1~10 μg线性关系均良好
加样回收率为98.91%~102.23%。 结论: 建立了重楼皂苷Ⅰ
Ⅱ
Ⅵ
Ⅶ及金丝桃苷的HPLC测定方法
操作方便
结果准确
可用于重楼克感胶囊中5种化合物的含量测定。
Abstract
Objective: A rapid and specific RP-HPLC-UV method was developed for the analysis of polyphyllin Ⅰ
Ⅱ
Ⅵ
Ⅶ and hyperoside. The method was applied to study the content determination of Chonglou Kegan capsule containing polyphyllin Ⅰ
Ⅱ
Ⅵ
Ⅶ and hyperoside. Method: The analysis was carried out on a Diamonsil C18 (2) reversed-phase column (4.6 mm×250 mm
5 μm) by isocratic elution with acetonitrile and 0.1% phosphoric acid (42:58). The flow rate was 1.0 mL·min-1 and the detection wavelength was set at 203
360 nm. Result: Linear responses were obtained for polyphyllin Ⅰ
Ⅱ
Ⅵ
Ⅶ and hyperoside ranging from 0.1 to 10 μg. The extraction recovery ranged from 98.91%-102.12%. Stability studies showed that polyphyllin Ⅰ
Ⅱ
Ⅵ
Ⅶ and hyperoside were stable in preparation and analytical process. Conclusion: The results indicated that the validated method was successfully used to determine the concentration of polyphyllin Ⅰ