Objective: To develop a HPLC method for the simultaneous determination of gastrodin

5-hydroxymethylfurfural and ferulic acid in Tianshu Capsule. Method: Phenomenex Luna C18 column (4.6 mm×250 mm

5 μm) was used. The mobile phase consisted of methanol (A) and water containing 0.1% phosphoric acid (B)

and the gradient program was as followed:0-10 min

5%-10%A;10-16 min

10%A;16-50 min

10%-60%A. The flow rate was 1.0 mL·min-1 and the column temperature was set at 30℃. The detection wavelength was at 221 nm (gastrodin)

283 nm (5-hydroxymethylfurfural) and 322 nm (ferulic acid). Result: The linear ranges were 5.61-299 mg·L-1 for gastrodin

3.31-212 mg·L-1 for 5-hydroxymethylfurfural and 4.63-247 mg·L-1 for ferulic acid

respectively. The average recoveries of three constituents were 99.74%

102.5% and 100.3% with the RSD of 0.84%

1.53% and 0.49%. The contents of three compounds

gastrodin

5-hydroxymethylfurfural and ferulic acid in Tianshu Capsules were 2.70-3.65

2.07-2.67 mg·g-1 and 1.95-2.10 mg·g-1. Conclusion: Determination of gastrodin

5-hydroxymethylfurfural and ferulic acid could effectively control the product quality of Tianshu Capsules.