Objective: To develop an HPLC method for the simultaneous determination of seven major compound in Xiaoaiping injection. Method: The analytical column was Kromasil 100-5C18(4.6 mm×250 mm

5 μm). The mobile phase was acetonitrile-0.2% phosphoric acid

gradient elution. The flow was 0.8 mL·min-1. The detection wavelength was 300 nm. The column temperature was 30 ℃. Result: The linear ranges of seven compounds were as fllows:0.1-3.2 mg·L-1 (r=0.999 3)for neochlorogenic acid

0.025-0.8 mg·L-1(r=0.999 9)for protocatechuic aldehyde

0.1-3.2 mg·L-1(r=0.999 8) for chlorogenic acid

0.075-2.4 mg·L-1(r=0.999 9) for 4-dicaffeoylquinic acid

0.037 5-1.2 mg·L-1(r=0.999 9) for vanillic acid

0.02-0.64 mg·L-1(r=0.999 9) for caffeic acid

0.01-0.32 mg·L-1(r=0.999 9)for 4-coumararate.The average recoveries were 99.6% (RSD 0.24%)

100.0% (RSD 0.15%)

98.7% (RSD 1.00%)

99.1% (RSD 1.36%)

96.4% (RSD 1.37%)

98.3% (RSD 1.80%)

97.3% (RSD 1.64%) respectively. Conclusion: This method is simple

accurate and available for the quality control of Xiaoaiping Injection.