槐米中槲皮素的双相动态提取工艺优选

李除夕; 丁菲; 贾树田; 胡正芳

doi:10.11653/syfj2013230001

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槐米中槲皮素的双相动态提取工艺优选

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- 槐米中槲皮素的双相动态提取工艺优选
- Optimization of Diphasic Dynamic Extraction Technology for Quercetin from Flos Sophorae Immaturus
- 中国实验方剂学杂志 2013年19卷第23期页码：1-3
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家"十一五"科技支撑计划课题(2006BAI06A00)
- DOI：10.11653/syfj2013230001
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- 纸质出版日期：2013
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李除夕, 丁菲, 贾树田, 等. 槐米中槲皮素的双相动态提取工艺优选[J]. 中国实验方剂学杂志, 2013,19(23):1-3.

LI Chu-xi, DING Fei, JIA Shu-tian, et al. Optimization of Diphasic Dynamic Extraction Technology for Quercetin from Flos Sophorae Immaturus[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(23): 1-3.
李除夕, 丁菲, 贾树田, 等. 槐米中槲皮素的双相动态提取工艺优选[J]. 中国实验方剂学杂志, 2013,19(23):1-3. DOI： 10.11653/syfj2013230001.

LI Chu-xi, DING Fei, JIA Shu-tian, et al. Optimization of Diphasic Dynamic Extraction Technology for Quercetin from Flos Sophorae Immaturus[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(23): 1-3. DOI： 10.11653/syfj2013230001.

摘要

目的: 优选槐米中槲皮素的双相动态提取工艺。方法: 以槲皮素提取量为评价指标，在单因素试验基础上，通过正交试验考察提取时间、提取温度、提取次数、硫酸体积分数对槲皮素双相动态提取工艺的影响。采用HPLC测定槲皮素含量，色谱条件为Agilent ZORBAX C18色谱柱（4.6 mm×250 mm，5 μm），流动相甲醇-0.02%H3PO4（45∶55），检测波长254 nm，进样量10 μL。结果: 槐米中槲皮素的最佳提取工艺为加2%硫酸于85℃提取2次，每次2.5 h;槲皮素提取量达5.22 mg·g-1，远高于乙醇回流提取法的3.26 mg·g-1。结论: 优选的提取工艺稳定可行，采用双相动态工艺提取槲皮素具有提取率高的优势。

Abstract

Objective: To optimize diphasic dynamic extraction technology of quercetin from Flos Sophorae Immaturus. Method: With the content of quercetin as index

based on single factor tests

L9(34) orthogonal test was adopted to optimize diphasic dynamic extraction technology of quercetin by taking extraction time

extraction temperature

extraction times and sulfuric acid concentration as factors.The content of quercetin was determined by HPLC

chromatographic conditions were as follows:Agilent ZORBAX C18 column(4.6 mm×250 mm

5 μm)

mobile phase of methanol-0.02% H3PO4(45:55)

detection wavelength 254 nm

injection volume 10 μL. Result: Optimal extraction technology was as following:extracted 2 times with 2% sulfuric acid at 85℃

2.5 hours per time;Yield of quercetin was up to 5.22 mg· g-1

which was more than ethanol extraction method of 3.26 mg· g-1. Conclusion: This optimized extraction technology was feasible and stable with high yield of quercetin.

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