正交试验优选蛇黄凝胶提取工艺

汪亚飞; 钟希文; 梅全喜; 张文霞; 王贤儿

doi:10.11653/syfj2013240039

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正交试验优选蛇黄凝胶提取工艺

更新时间：2024-01-04

- 正交试验优选蛇黄凝胶提取工艺
- Optimization of Extraction Technology for Shehuang Gels by Orthogonal Design
- 中国实验方剂学杂志 2013年19卷第24期页码：39-42
- 作者机构：
- 作者简介：
- 基金信息：
  
  广东省中山市科技计划项目（20102A031）
- DOI：10.11653/syfj2013240039
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
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汪亚飞, 钟希文, 梅全喜, 等. 正交试验优选蛇黄凝胶提取工艺[J]. 中国实验方剂学杂志, 2013,19(24):39-42.

WANG Ya-fei, ZHONG Xi-wen, MEI Quan-xi, et al. Optimization of Extraction Technology for Shehuang Gels by Orthogonal Design[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(24): 39-42.
汪亚飞, 钟希文, 梅全喜, 等. 正交试验优选蛇黄凝胶提取工艺[J]. 中国实验方剂学杂志, 2013,19(24):39-42. DOI： 10.11653/syfj2013240039.

WANG Ya-fei, ZHONG Xi-wen, MEI Quan-xi, et al. Optimization of Extraction Technology for Shehuang Gels by Orthogonal Design[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(24): 39-42. DOI： 10.11653/syfj2013240039.

摘要

目的：优选蛇黄凝胶的提取工艺。方法：采用L9（34）正交试验设计，以三羽新月蕨苷A、大黄素和干膏得率为评价指标，在单因素试验基础上，通过综合评分优选蛇黄凝胶的提取工艺。采用HPLC测定三羽新月蕨苷A、大黄素含量，流动相乙腈（A）-0.1%磷酸水（B）-水（C）梯度洗脱（0~40 min，10%~70% A，10% B，80%~20%C），检测波长226 nm。结果：最佳提取工艺条件为加14倍量70%乙醇提取3次，每次30 min；大黄素、三羽新月蕨苷A平均提取量依次为0.37，0.76 mg·g-1。结论：优选的提取工艺稳定可行，对蛇黄凝胶的生产工艺具有一定的指导和参考意义。

Abstract

Objective: To optimize extraction technology of Shehuang gels by orthogonal test. Method: L9(34) Orthogonal test was performed with extraction amounts of triphyllin A and emodin

dry extract yield as indexes

based on single factor tests

extraction process of Shehuang gels was optimized by multi-index comprehensive evaluation method.Contents of triphyllin A and emodin were determined by HPLC

mobile phase of acetonitrile(A)-0.1% phosphoric acid(B)-water(C) with a gradient elution program(0-40 min

10%-70% A

10% B

80%-20%C)

detection wavelength 226 nm. Result: Optimal extraction conditions were as follows:extracted 3 times with 14 times the amount of 70% ethanol

30 min for each time;Average extraction amounts of triphyllin A and emodin were 0.37

0.76 mg·g-1. Conclusion: This optimized technology was stable and feasible

which had some guidance and reference value for production of Shehuang gels.

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