HPLC同时测定复方黄连素片中3种成分的含量

刘晓哲; 张东方

doi:10.11653/syfj2014010070

您当前的位置：

首页 >

文章列表页 >

HPLC同时测定复方黄连素片中3种成分的含量

更新时间：2024-01-04

- HPLC同时测定复方黄连素片中3种成分的含量
- Simultaneous Determination of Peoniflorin, Costunolide, Dehydrocostus Lactone in Compound Berberine Tablets by HPLC
- 中国实验方剂学杂志 2014年20卷第1期页码：70-73
- 作者机构：
- 作者简介：
- 基金信息：
  
  河南省科技厅重点科技攻关项目（112102210030）
- DOI：10.11653/syfj2014010070
  中图分类号：
- 纸质出版日期：2014
- 稿件说明：
移动端阅览
刘晓哲, 张东方. HPLC同时测定复方黄连素片中3种成分的含量[J]. 中国实验方剂学杂志, 2014,20(1):70-73.

LIU Xiao-zhe, ZHANG Dong-fang. Simultaneous Determination of Peoniflorin, Costunolide, Dehydrocostus Lactone in Compound Berberine Tablets by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(1): 70-73.
刘晓哲, 张东方. HPLC同时测定复方黄连素片中3种成分的含量[J]. 中国实验方剂学杂志, 2014,20(1):70-73. DOI： 10.11653/syfj2014010070.

LIU Xiao-zhe, ZHANG Dong-fang. Simultaneous Determination of Peoniflorin, Costunolide, Dehydrocostus Lactone in Compound Berberine Tablets by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(1): 70-73. DOI： 10.11653/syfj2014010070.

摘要

目的：同时测定复方黄连素片中芍药苷、木香烃内酯、去氢木香内酯3种成分的含量。方法：采用高效液相色谱法，C18柱，以乙腈（A）-0.2%的磷酸溶液（B）为流动相进行梯度洗脱，流速1.0 mL·min-1，检测波长225 nm，柱温30℃。结果：芍药苷在0.180 9~3.618 μg线性关系良好（r=0.999 8），平均加样回收率为99.0%，RSD 0.94%；木香烃内酯在0.129 85~2.597 μg线性关系良好（r=0.999 7），平均加样回收率为98.4%，RSD 1.62%；去氢木香内酯在0.120 8~2.416 μg线性关系良好（r=0.999 6），平均加样回收率为97.9%，RSD 1.21%。结论：该方法简便、准确，可用于测定复方黄连素片的含量。

Abstract

Objective: To establish a method for simultaneous determining peoniflorin

costunolide and dehydrocostus lactone in compound berberine tablets. Method: Three components were separated by C18 chromatographic column with a gradient mobile phase consisting of acetonitrile and 0.2% phosphoric acid solution at a flow rate of 1.0 mL·min-1

detection wavelength was 225 nm

and column temperature of 30℃. Result: The linear ranges of peoniflorin

costunolide

and dehydrocostus lactone fell within the ranges of 0.180 9-3.618

0.129 85-2.597

0.120 8-2.416 μg

respectively. The three components showed good linear correlations (r=0.999 8

0.999 7

0.999 6).The recoveries were 99.0% for peoniflorin

98.4% for costunolide

97.9% for dehydrocostus lactone

respectively. The relative standard deviations were 0.94%

1.62% and 1.21%. Conclusion: The method is simple and accurate. It can be used for quality control of compound berberine tablets.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

超临界流体色谱分离纯化木香中的木香烃内酯和去氢木香内酯

HPLC测定维药金锁昆都尔片中木香含量

木香中去氢木香内酯和木香烃内酯提取工艺优选

煨木香醇提物的纯化工艺优选