硫酸-紫外法与苯酚-硫酸法测定千两茶中总多糖的比较

辛敏; 刘轩; 詹欣; 黄昀; 唐劲天; 盛军

doi:10.11653/syfj2014030062

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硫酸-紫外法与苯酚-硫酸法测定千两茶中总多糖的比较

更新时间：2024-01-04

- 硫酸-紫外法与苯酚-硫酸法测定千两茶中总多糖的比较
- Determination of Total Polysaccharides in Qianliang Tea by Sulfuric Acid-UV Method and Phenol-Sulfuric Acid Method
- 中国实验方剂学杂志 2014年20卷第3期页码：62-65
- 作者机构：
- 作者简介：
- 基金信息：
  
  中国博士后基金项目（2013M530603）
- DOI：10.11653/syfj2014030062
  中图分类号：
- 纸质出版日期：2014
- 稿件说明：
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辛敏, 刘轩, 詹欣, 等. 硫酸-紫外法与苯酚-硫酸法测定千两茶中总多糖的比较[J]. 中国实验方剂学杂志, 2014,20(3):62-65.

XIN Min, LIU Xuan, ZHAN Xin, et al. Determination of Total Polysaccharides in Qianliang Tea by Sulfuric Acid-UV Method and Phenol-Sulfuric Acid Method[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(3): 62-65.
辛敏, 刘轩, 詹欣, 等. 硫酸-紫外法与苯酚-硫酸法测定千两茶中总多糖的比较[J]. 中国实验方剂学杂志, 2014,20(3):62-65. DOI： 10.11653/syfj2014030062.

XIN Min, LIU Xuan, ZHAN Xin, et al. Determination of Total Polysaccharides in Qianliang Tea by Sulfuric Acid-UV Method and Phenol-Sulfuric Acid Method[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(3): 62-65. DOI： 10.11653/syfj2014030062.

摘要

目的：比较苯酚-硫酸法与硫酸-紫外法在测定千两茶多糖含量上的差异。方法：千两茶粉经水提醇沉，Sevage法除蛋白后，采用苯酚-硫酸法和硫酸-紫外法测定茶多糖含量。结果：苯酚-硫酸法标准曲线方程为Y=4.755 0X-0.029 5（R2=0.990 4），硫酸-紫外法标准曲线方程为Y=4.770 0X-0.065 1（R2=0.993 1），均在0.02~0.1 g·L-1有良好的线性关系。所测茶多糖质量分数分别为2.00%，3.21%，其差异有统计学意义。结论：硫酸-紫外法作为一种多糖检测新手段，可简便测定多糖含量。

Abstract

Objective: To compare differences between phenol-sulfuric acid method and sulfuric acid-UV method to determine polysaccharides content in Qianliang tea. Method: The two methods were employed to determine content of polysaccharides in Qianliang tea after water extracting-alcohol precipitating and sevage assays. Result: The total sugar stand curve equation were Y=4.755 0X-0.029 5(R2=0.990 4)(by sulfuric acid-UV method) and Y=4.770 0X-0.065 1(R2=0.993 1)(by phenol-sulfuric acid method)

which showed a good linear relationship range from 0.02 to 0.1 g·L-1. The determination of phenol-sulfuric acid method and sulfuric acid-UV method were 2.00% and 3.21%

which was statistically significance. Conclusion: As a new method

sulfuric acid-UV method could be a stable and simple method to measure the polysaccharide.

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