土知母药材及其混淆品HPLC指纹图谱研究

冯华; 聂明华; 罗秀琼; 王祥培

doi:10.11653/syfj2014060067

您当前的位置：

首页 >

文章列表页 >

土知母药材及其混淆品HPLC指纹图谱研究

更新时间：2024-01-04

- 土知母药材及其混淆品HPLC指纹图谱研究
- HPLC Fingerprints of and its Adulterants from Different Places
- 中国实验方剂学杂志 2014年20卷第6期页码：67-69
- 作者机构：
- 作者简介：
- 基金信息：
  
  贵州省中药现代化科技产业研究开发专项项目（黔科合中药字[2010]5028号）
- DOI：10.11653/syfj2014060067
  中图分类号：
- 纸质出版日期：2014
- 稿件说明：
移动端阅览
冯华, 聂明华, 罗秀琼, 等. 土知母药材及其混淆品HPLC指纹图谱研究[J]. 中国实验方剂学杂志, 2014,20(6):67-69.

FENG Hua, NIE Ming-hua, LOU Xiu-qiong, et al. HPLC Fingerprints of and its Adulterants from Different Places[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(6): 67-69.
冯华, 聂明华, 罗秀琼, 等. 土知母药材及其混淆品HPLC指纹图谱研究[J]. 中国实验方剂学杂志, 2014,20(6):67-69. DOI： 10.11653/syfj2014060067.

FENG Hua, NIE Ming-hua, LOU Xiu-qiong, et al. HPLC Fingerprints of and its Adulterants from Different Places[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(6): 67-69. DOI： 10.11653/syfj2014060067.

摘要

目的：研究并建立土知母药材的指纹图谱。方法：采用高效液相色谱法，Diamonsil C18柱（4.6 mm×200 mm，5 μm），乙腈-0.2磷酸溶液进行梯度洗脱，检测波长275 nm，柱温30 ℃，对19个不同来源的药材进行了检测。结果：建立了土知母药材HPLC指纹图谱共有模式，15个共有峰被标定，对19批土知母及混淆品进行了相似度比较。12批土知母药材的相似度均>0.90，7批混淆品均<0.65。结论：HPLC指纹图谱鉴别土知母及混淆品具有重复性好、特征性强、方法简便等特点，可作为土知母药材鉴别方法。

Abstract

Objective: To study and establish the fingerprints of Iris tectorum by HPLC. Method: The fingerprints of I.tectorum was built by using DiamonsilTMC18(4.6 mm×200 mm

5 μm) as analytical column and acetonitrile-0.2% phosphoric acid as mobile phase. The flow rate was 1.0 mL·min-1. Detecting wavelength was set at 275 nm. The temperature of column was at 30 ℃.Total 19 batches of I.tectorum samples and common peaks were pinpointed analyzed. Result: The mutual mode to HPLC fingerprints was set up

and the 15 sources were obtained.The similarities in 12 batches of I.tectorum samples were all above 0.90 and those of 7 batches of adulterants were below 0.65. Conclusion: The method is simple

stable and repeoducible

which can be uesd to identify I.tectorum and its adulterants.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

菊花配方颗粒的HPLC指纹图谱研究

三七配方颗粒的HPLC指纹图谱研究

湖北海棠HPLC指纹图谱的研究

布渣叶药材HPLC指纹图谱研究