Objective: To establish the fingerprint method of Herba Bidentis and identify five species of Herb Bidens. Method: The methanol extraction of Herba Bidentis was separated on a Kromasil C18 column(4.6 mm×250 mm

5 μm) eluted with Methanol-(0.1%phosphoric) acid water in linear gradient condition with DAD detected at 290 nm.Flow rate was 1 mL·min-1 and 10 μL was injected and the column temperature was 30 ℃. Result: The fingerprint of Herba Bidentis was established for 10 samples of different sources. Principal components analysis and clustering analysis were used in the identification of the samples

and the similitude was computed by computer. Conclusion: It was also the simple extraction and the most chemical components could be separated very well by HPLC. This method was stable

repeatable and feasible. And it provided scientific reference and effective method for the quality control of Herba Bidentis. The fingerprint could be used to distinguish Herba Bidentis.