Objective: To analyze the chemical constituents and establish a HPLC fingerprint of flavonoids parts of Smilax glabra. Method: UPLC-UV-MS technology was used to identify the chemical constituents of flavonoids parts of S. glabra; Diamonsil C18 column (4.6 mm×250 mm

5 μm)

methanol and 1.5% acetic acid solvent system gradient elution were applied

with flow rate of 0.8 mL·min-1 and UV detector at 280 nm. The column temperature was maintained at 30 ℃ and 10 μL was injected every time. HPLC method was used to establish a fingerprint. Result: Sixteen compounds were identified

among them

9 chemical constituents were identified by direct comparison with the reference substances; in the fingerprint

24 common peaks were confirmed. The results of similarity analysis of 10 batches of flavonoids parts of Smilax Glabra from different sources were above 0.99. Conclusion: The constituents of flavonoids parts of S. Glabra were clear. HPLC fingerprint has good repeatability

precision

strong specificity

and can be used to control the quality of flavonoids parts of S. Glabra. This research laid a foundation for further studies on pharmacodynamic material basis of flavonoids parts of Smilax Glabra.