正交试验法研究补阳还五汤中保护OGD损伤PC12细胞的主要药味

张继平; 刘俊娥; 姚晖; 方永奇; 李翎; 文凤妮; 朱永坤

doi:10.13422/j.cnki.syfjx.2014110134

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正交试验法研究补阳还五汤中保护OGD损伤PC12细胞的主要药味

药理 | 更新时间：2024-01-04

- 正交试验法研究补阳还五汤中保护OGD损伤PC12细胞的主要药味
- Main Ingredients of Buyang Huanwu Decoction in Protecting PC12 Cells During OGD by Orthogonal Test
- 中国实验方剂学杂志 2014年20卷第11期页码：134-138
- 作者机构：
- 作者简介：
- 基金信息：
  
  广东省中药局中医药强省项目（2010008，20111058，20121088）
- DOI：10.13422/j.cnki.syfjx.2014110134
  中图分类号： R285.5
- 纸质出版日期：2014
- 稿件说明：
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张继平, 刘俊娥, 姚晖, 等. 正交试验法研究补阳还五汤中保护OGD损伤PC12细胞的主要药味[J]. 中国实验方剂学杂志, 2014,20(11):134-138.

ZHANG Ji-ping, LIU Jun-e, YAO Hui, et al. Main Ingredients of Buyang Huanwu Decoction in Protecting PC12 Cells During OGD by Orthogonal Test[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(11): 134-138.
张继平, 刘俊娥, 姚晖, 等. 正交试验法研究补阳还五汤中保护OGD损伤PC12细胞的主要药味[J]. 中国实验方剂学杂志, 2014,20(11):134-138. DOI： 10.13422/j.cnki.syfjx.2014110134.

ZHANG Ji-ping, LIU Jun-e, YAO Hui, et al. Main Ingredients of Buyang Huanwu Decoction in Protecting PC12 Cells During OGD by Orthogonal Test[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(11): 134-138. DOI： 10.13422/j.cnki.syfjx.2014110134.

摘要

目的：研究补阳还五汤中保护缺氧缺糖（oxygen-glucose deprivation，OGD）损伤PC12细胞的主要药味。方法：将补阳还五汤中7种单味药（黄芪、赤芍、当归尾、地龙、川芎、桃仁、红花）作为实验因素，每个因素选取有或无2个水平，按L8（27）正交试验表设计8种拆方。普通级新西兰兔分为9组，空白血清组和8个拆方含药血清组，分别ig蒸馏水和临床等效剂量5倍的拆方药液，24 h内连续ig 3次后，提取血清。运用四甲基偶氮唑盐（methyl thiazolyl tetrazolium，MTT）和微量酶标法，以细胞活力和乳酸脱氢酶（lactate dehydrogenase，LDH）释放量为指标，观察10%补阳还五汤及其拆方含药血清对OGD损伤18 h的PC12细胞的影响。结果：PC12细胞OGD损伤后，其LDH释放量增加（P<0.01），补阳还五汤能显著抑制OGD损伤PC12细胞的LDH释放量（P<0.01）。正交试验结果显示，含黄芪、当归尾、赤芍的拆方组其细胞活力显著增加（P<0.01），LDH释放量显著降低（P<0.01）；而含桃仁的拆方组其细胞活力显著下降（P<0.01），含地龙的拆方组其LDH释放量显著升高（P<0.01）；红花、川芎对细胞活力及LDH释放量的作用不明显。结论：补阳还五汤对OGD损伤PC12细胞具有保护作用，其中黄芪、当归尾、赤芍是保护OGD损伤PC12细胞的主要药味，桃仁、地龙可加重PC12细胞OGD损伤，红花、川芎对细胞的作用不明显。

Abstract

Objective:To investigate the main ingredients of Buyang Huanwu decoction(BYHWD) in protecting PC12 cells during oxygen-glucose deprivation(OGD). Method:Seven factors were selected from seven ingredients of BYHWD:Astragali Radix

Paeoniae Radix Rubra

Angelica Sinensis Radix Tail

Pheretima

Chuanxiong Rhizoma

Persicae Semen and Carthami Flos. Each factor was divided into two levels

contained or not contained. The experiment was arranged according to L8(27) orthogonal test table. Conventional New Zealand rabbits were divided into 9 groups randomly

rabbits of the blank control group were fed with distilled water

rabbits of the other eight groups were fed with eight prescriptions. After three times a day feeding

serum were prepared. PC12 cells were cultured with 10% serum during OGD for 18 hours. Viability and lactate dehydrogenase(LDH) leakage was detected by methyl thiazolyl tetrazolium(MTT) and micro enzyme-linked immunosorbent assay. Result:The increased LDH leakage was observed on PC12 cells during OGD(P<0.01). BYHWD could markedly decrease the LDH leakage(P<0.01). Orthogonal analysis showed

PC12 cell viability of prescription with Astragali Radix

Paeoniae Radix Rubra and Angelica Sinensis Radix Tail was significantly increased(P<0.01)

LDH leakage of prescription with Astragali Radix

Paeoniae Radix Rubra and Angelica Sinensis Radix Tail was significantly decreased(P<0.01). PC12 cell viability of prescription with Persicae Semen was reduced(P<0.01). PC12 cell LDH leakage of prescription with Pheretima was increased(P<0.01). Effect of other medicines was not obvious. Conclusion:BYHWD has protective effects on PC12 cells during OGD. Astragali Radix

Paeoniae Radix Rubra and Angelica

Sinensis Radix Tail play a major role. Persicae Semen and Pheretima can aggravate cell injury. Carthami Flos and Rhizoma has not obvious effect on PC12 cells during OGD.

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