二神丸中药物炮制前后化学成分含量变化

耿媛媛; 胡昌江; 潘新; 胡麟; 赵玲; 熊瑞

doi:10.13422/j.cnki.syfjx.2014130117

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二神丸中药物炮制前后化学成分含量变化

化学与分析 | 更新时间：2024-01-04

- 二神丸中药物炮制前后化学成分含量变化
- Contents Change of Chemical Compositions in Ershen Pills Before and After Preparation
- 中国实验方剂学杂志 2014年20卷第13期页码：117-120
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（81274085）
- DOI：10.13422/j.cnki.syfjx.2014130117
  中图分类号： R286.0
- 纸质出版日期：2014
- 稿件说明：
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耿媛媛, 胡昌江, 潘新, 等. 二神丸中药物炮制前后化学成分含量变化[J]. 中国实验方剂学杂志, 2014,20(13):117-120.

GENG Yuan-yuan, HU Chang-jiang, PAN Xin, et al. Contents Change of Chemical Compositions in Ershen Pills Before and After Preparation[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(13): 117-120.
耿媛媛, 胡昌江, 潘新, 等. 二神丸中药物炮制前后化学成分含量变化[J]. 中国实验方剂学杂志, 2014,20(13):117-120. DOI： 10.13422/j.cnki.syfjx.2014130117.

GENG Yuan-yuan, HU Chang-jiang, PAN Xin, et al. Contents Change of Chemical Compositions in Ershen Pills Before and After Preparation[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(13): 117-120. DOI： 10.13422/j.cnki.syfjx.2014130117.

摘要

目的：建立同时测定二神丸中3种成分含量的HPLC，比较二神丸中药物炮制前后补骨脂素、异补骨脂素和去氢二异丁香酚含量的变化。拟阐明炮制对方中药物化学成分的影响。方法：二神丸中的药物补骨脂盐炙，肉豆蔻麸煨，采用HPLC测定2味药炮制前后3种成分的含量变化。结果：该测定方法补骨脂素的线性范围0.164~2.05 μg，回收率101.76%，RSD 2.02%；异补骨脂素的线性范围0.115~1.44 μg，回收率96.15%，RSD 1.55%；去氢二异丁香酚的线性范围0.086 4~1.08 μg，回收率99.61%，RSD 1.96%。二神丸中药物炮制后，补骨脂素和异补骨脂素的含量均降低，去氢二异丁香酚的含量亦降低。结论：该方法简单准确，重复性好。二神丸中药物炮制后补骨脂素、异补骨脂素和去氢二异丁香酚的含量均发生了一定变化。

Abstract

Objective: To establish an HPLC method for simultaneous determination of three components in Ershen pills and compare the content changes of psoralen

isopsoralen and dehydrodiisoeugenol in this prescription before and after processing

and to investigate the influence of processing on chemical compositions. Method: Psoralea was stir-fried with salt and Nutmeg was roasted with wheat bran.HPLC was applied to determine the content changes of psoralen

isopsoralen and dehydrodiisoeugenol in this prescription before and after processing. Result: The average recoveries of psoralen

isopsoralen and dehydrodiisoeugenol were 101.76%

96.15%

99.61%

with RSD being 2.02%

1.55%

1.96%.The psoralen

isopsoralen and dehydrodiisoeugenol samples showed a good linear relationship in the ranges of 0.164-2.05

0.115-1.44

0.086 4-1.08 μg

respectively

in the chosen HPLC conditions.After preparation

the three compositions were reduced. Conclusion: The method is simple

accurate and reproducible. The contents of psoralen

isopsoralen and dehydrodiisoeugenol in Ershen pills can be changed after processing.

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