肉苁蓉苯乙醇苷对大鼠高原肺水肿的防治作用

陶义存; 李建英; 许永华; 靳春丽; 屠鹏飞; 毛新民

doi:10.13422/j.cnki.syfjx.2014150134

您当前的位置：

首页 >

文章列表页 >

肉苁蓉苯乙醇苷对大鼠高原肺水肿的防治作用

药理 | 更新时间：2024-01-04

- 肉苁蓉苯乙醇苷对大鼠高原肺水肿的防治作用
- Preventive Effect of Phenylethanoid Glycosides from Cistanches Herba on Rats with High Altitude Pulmonary Edema
- 中国实验方剂学杂志 2014年20卷第15期页码：134-138
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（81160416）
- DOI：10.13422/j.cnki.syfjx.2014150134
  中图分类号： R285.5
- 纸质出版日期：2014
- 稿件说明：
移动端阅览
陶义存, 李建英, 许永华, 等. 肉苁蓉苯乙醇苷对大鼠高原肺水肿的防治作用[J]. 中国实验方剂学杂志, 2014,20(15):134-138.

TAO Yi-cun, LI Jian-ying, XU Yong-hua, et al. Preventive Effect of Phenylethanoid Glycosides from Cistanches Herba on Rats with High Altitude Pulmonary Edema[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(15): 134-138.
陶义存, 李建英, 许永华, 等. 肉苁蓉苯乙醇苷对大鼠高原肺水肿的防治作用[J]. 中国实验方剂学杂志, 2014,20(15):134-138. DOI： 10.13422/j.cnki.syfjx.2014150134.

TAO Yi-cun, LI Jian-ying, XU Yong-hua, et al. Preventive Effect of Phenylethanoid Glycosides from Cistanches Herba on Rats with High Altitude Pulmonary Edema[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(15): 134-138. DOI： 10.13422/j.cnki.syfjx.2014150134.

摘要

目的：观察肉苁蓉苯乙醇苷预防给药对高原肺水肿模型大鼠的作用及可能的作用机制。方法：正常对照组，模型组，大花红景天口服液组（1.78 mL·kg-1·d-1），肉苁蓉苯乙醇苷低、中、高剂量组（75，150，300 mg·kg-1·d-1），每组12只，灌胃预防给药10 d，第8天起除空白对照组外其余各组置于模拟海拔5 000 m高原环境72 h建立高原肺水肿模型，观察各组大鼠肺组织病理改变，检测肺组织含水量、肺组织匀浆中白介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）、丙二醛（MDA）含量及超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）酶活力。结果：与正常对照组[肺组织含水量为（77.38±0.67）%，肺匀浆IL-6含量（181.49±26.00） ng·L-1，TNF-α含量（83.33±33.31）ng·L-1，MDA含量（5.25±1.13） nmol·mg-1，SOD酶活力（124.74±35.45）U·mg-1，GSH-Px酶活力（234.16±17.33） U·mg-1]相比，模型组大鼠出现明显的肺水肿，肺组织含水量显著升高、肺匀浆液中IL-6，TNF-α，MDA含量增高，SOD和GSH-Px酶活力显著降低。与模型组比较，肉苁蓉苯乙醇苷能够改善高原肺水肿大鼠肺水肿病理改变，降低肺组织含水量，降低肺匀浆中IL-6，TNF-α，MDA含量且提高SOD和GSH-Px的酶活力。结论：肉苁蓉苯乙醇苷能够预防高原肺水肿的发生，与其抗炎、抗氧化应激有关。

Abstract

Objective: To observe the effect and mechanism of phenylethanoid glycosides from Cistanches Herba on rat with high-altitude pulmonary edema. Method: Phenylethanoid glycosides from Cistanche Herba was given prophylactically to estibalish the rats model of high-altitude pulmonary edema by using hypobaric chamber in a simulated environment of 5000m altitude. The pathologic changes of rat lung tissue

the water content

the content of interleukin 6 (IL-6)

tumor necrosis factor-α(TNF-α)

malondialdehyde(MDA) and enzymatic activity of superoxide dismutase(SOD)

glutathione peroxidase(GSH-Px) in lung homogenate were measured. Result: Compared with the control group

the high altitude pulmonary edema model rats showed the remarkable character of pulmonary edema and the higher content of water in lung. The content of IL-6

TNF-α

MDA in lung homogenate were increased while enzymatic activity of SOD

GSH-Px were significantly decreased. The phenylethanoid glycosides from Cistanches Herba were able to improve pathological change of lung tissue

decrease water content

IL-6

TNF-α

MDA while increase enzymatic activity of SOD

GSH-Px in lung homogenate. Conclusion: Phenylethanoid glycosides from Cistanches Herba were able to prevent the high altitude pulmonary edema

the mechanism of which may related with the anti-inflammation

anti-oxidative stress in lung tissue.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

肉苁蓉不同提取部位改善肾阳虚大鼠性能力的影响

肉苁蓉高品质形成的研究进展

基于UPLC-Q-TOF/MS^E分析不同炮制时间炒车前子的化学成分变化规律

肉苁蓉尿囊素提取物安全性毒理学评价