天麻与钩藤配伍前后效应成分在SHR大鼠脑组织的分布

米佳; 王兴; 董雨; 李莹

doi:10.13422/j.cnki.syfjx.2014180105

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天麻与钩藤配伍前后效应成分在SHR大鼠脑组织的分布

药物代谢 | 更新时间：2024-01-04

- 天麻与钩藤配伍前后效应成分在SHR大鼠脑组织的分布
- Distribution of Gastrodigenin from Gastrodiae Rhizoma in Brain Tissue of SHR Rats Before and After Compatibility with Uncariae Ramulus Cum Uncis
- 中国实验方剂学杂志 2014年20卷第18期页码：105-108
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（30801547）;四川省科技支撑计划项目（2013SZ0130）
- DOI：10.13422/j.cnki.syfjx.2014180105
  中图分类号： R965
- 纸质出版日期：2014
- 稿件说明：
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米佳, 王兴, 董雨, 等. 天麻与钩藤配伍前后效应成分在SHR大鼠脑组织的分布[J]. 中国实验方剂学杂志, 2014,20(18):105-108.

MI Jia, WANG Xing, DONG Yu, et al. Distribution of Gastrodigenin from Gastrodiae Rhizoma in Brain Tissue of SHR Rats Before and After Compatibility with Uncariae Ramulus Cum Uncis[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(18): 105-108.
米佳, 王兴, 董雨, 等. 天麻与钩藤配伍前后效应成分在SHR大鼠脑组织的分布[J]. 中国实验方剂学杂志, 2014,20(18):105-108. DOI： 10.13422/j.cnki.syfjx.2014180105.

MI Jia, WANG Xing, DONG Yu, et al. Distribution of Gastrodigenin from Gastrodiae Rhizoma in Brain Tissue of SHR Rats Before and After Compatibility with Uncariae Ramulus Cum Uncis[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(18): 105-108. DOI： 10.13422/j.cnki.syfjx.2014180105.

摘要

目的：建立SHR大鼠脑组织中天麻苷元的含量测定方法，考察天麻与钩藤配伍前后效应成分天麻苷元在脑组织中的分布情况。方法：采用HPLC测定脑组织中天麻苷元含量，流动相乙腈-0.03%磷酸溶液（7：93），检测波长221 nm。检测天麻与钩藤配伍前后效应成分天麻苷元在脑组织中的分布情况。结果：大鼠血浆中天麻苷元的线性范围0.5~100 mg·L-1（R2=0.999 9），方法回收率>74.89%，准确度>98%。配伍前、后天麻苷元在大鼠脑组织的药代动力学参数Cmax分别为0.485，0.373 μg·g-1，AUC依次为111.366，213.585 μg·g-1·min。结论：该方法能准确、灵敏测定大鼠脑组织中天麻苷元含量。天麻与钩藤配伍对天麻苷元在SHR大鼠脑组织中分布具有先抑制后促进影响。

Abstract

Objective: To establish an HPLC method for determination of gastrodigenin content in brain tissue of SHR rats and investigate its tissue distribution before and after compatibility of Gastrodiae Rhizoma and Uncariae Ramulus Cum Uncis. Method: HPLC was employed to determine the content of gastrodigenin in brain tissue with mobile phase of acetonitrile-0.03%H3PO4 (7:93) and detection wavelength at 221 nm.Distribution of gastrodigenin in brain tissue before and after compatibility of Gastrodiae Rhizoma and Uncariae Ramulus Cum Uncis was detected. Result: The linear range of calibration curve was within drug concentrations of 0.5-100 mg·L-1(R2=0.999 9)

recoveries of three levels were more than 74.89% and accuracies were more than 98%.Pharmacokinetic parameters of gastrodigenin in brain tissue before and after compatibility were received

such as Cmax were 0.485

0.373μg·g-1

AUC were 111.366

213.585 μg·g-1·min

respectively. Conclusion: This study establish a sensitive and accurate method for determination of gastrodigenin in brain tissue of SHR rats.Compatibility of Gastrodiae Rhizoma and Uncariae Ramulus Cum Uncis show a significant effect that first inhibiting and then promoting on distribution of gastrodigenin in brain tissue.

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