消脂汤对非酒精性脂肪性肝病模型大鼠治疗的作用及机制

杨道炬; 李常青; 廖向彬; 袁冬生

doi:10.13422/j.cnki.syfjx.2014180159

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消脂汤对非酒精性脂肪性肝病模型大鼠治疗的作用及机制

药理 | 更新时间：2024-01-04

- 消脂汤对非酒精性脂肪性肝病模型大鼠治疗的作用及机制
- Effects of Xiaozhi Decoction on SREBP-1c mRNA and Protein Expression in Hepatic Tissues of Rats with Non-alcoholic Fatty Liver Disease
- 中国实验方剂学杂志 2014年20卷第18期页码：159-164
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- DOI：10.13422/j.cnki.syfjx.2014180159
  中图分类号： R285.5
- 纸质出版日期：2014
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杨道炬, 李常青, 廖向彬, 等. 消脂汤对非酒精性脂肪性肝病模型大鼠治疗的作用及机制[J]. 中国实验方剂学杂志, 2014,20(18):159-164.

YANG Dao-ju, LI Chang-qing, LIAO Xiang-bin, et al. Effects of Xiaozhi Decoction on SREBP-1c mRNA and Protein Expression in Hepatic Tissues of Rats with Non-alcoholic Fatty Liver Disease[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(18): 159-164.
杨道炬, 李常青, 廖向彬, 等. 消脂汤对非酒精性脂肪性肝病模型大鼠治疗的作用及机制[J]. 中国实验方剂学杂志, 2014,20(18):159-164. DOI： 10.13422/j.cnki.syfjx.2014180159.

YANG Dao-ju, LI Chang-qing, LIAO Xiang-bin, et al. Effects of Xiaozhi Decoction on SREBP-1c mRNA and Protein Expression in Hepatic Tissues of Rats with Non-alcoholic Fatty Liver Disease[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(18): 159-164. DOI： 10.13422/j.cnki.syfjx.2014180159.

摘要

目的：观察消脂汤对非酒精性脂肪性肝病（NAFLD）模型大鼠的治疗作用，并探讨其机制。方法： 62只雄性SPF级SD大鼠，随机分为造模组（51只）和正常组（11只）。采用高脂饮食法复制大鼠NAFLD模型，造模成功后随机均匀分为模型组、非诺贝特组（0.05 g·kg-1·d-1）、消脂汤高、中、低剂量组（42.5，21.5，10.6 g·kg-1·d-1）。消脂汤组和非诺贝特组灌喂相应药物，正常组和模型组灌喂等体积生理盐水。4周后处死动物，全自动生化仪检测空腹血糖（FBG）、总胆固醇（TG）、甘油三酯（TC）、丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、谷氨酰胺转肽酶（GGT）、丙二醛（MDA）、超氧化物歧化酶（SOD）、游离脂肪酸（FFA），放射免疫法检测胰岛素（INS），计算胰岛素抵抗指数（HOMAR-IR），HE染色观察肝脏病理变化。RT-PCR方法检测肝组织固醇调节元件结合蛋白（SREBP-1c）mRNA的表达水平；Western blot方法检测肝组织SREBP-1c的蛋白表达水平。结果：与正常组比较，模型组血脂，肝脂，FFA，FINS，HOMAR-IR，MDA，SREBP-1c mRNA及蛋白表达显著升高（P<0.05或P<0.01）；SOD含量显著下降（P<0.01）。与模型组比较，各治疗组血脂，肝功，肝脂，FFA，FINS，HOMAR-IR，MDA，SREBP-1c mRNA及蛋白表达水平显著降低（P<0.05或P<0.01），以消脂汤高剂量组尤为显著（P<0.01）；SOD含量显著升高（P< 0.05或P<0.01）。结论：消脂汤能显著改善NAFLD的胰岛素抵抗和氧化应激，减轻肝脏脂肪蓄积和炎症反应，下调SREBP-1c mRNA和蛋白表达是其减轻肝脏脂肪蓄积的可能机制之一。

Abstract

Objective: To observe the effects of Xiaozhi decoction on sterol regulatory element-blinding protrin-1c (SREBP-1c) mRNA and SREBP-1c protein expression levels in hepatic tissue of rats with non-alcoholic fatty liver disease(NAFLD) and to explore the molecular mechanism. Method: NAFLD model was established by feeding high fat diet for 8 weeds. Then model rats were randomly divided into 5 groups:model group

fenofibrate group

Xiaozhi decoction group(high-dose group

dose group

low-dose group)

and additional normal control group. All rats were given normal diet. Meanwhile

rats in medication groups were fed with corresponding herbs

others were fed with corresponding NS. Four weeks after the treatment

all rats were anesthetized by 3% pentobarbital sodium through intraperitoneal injection. total cholesterol(TC)

triglyceride(TG) content in liver tissue and TC

glutamine transpeptidase(GGT)

aspartase aminotransferase(AST)

alanine aminotransferase(ALT)

content in the serum was detected by automatic biochemical analyzer. The pathological changes of hepatic tissue were observed by HE staining.The sterol regulatory element-blinding protein-1c (SREBP-1c)mRNA expression levels in liver tissue was examined by reverse transcription polymerase chain reaction(RT-PCR). The SREBP-1c protein expression levels in hepatic tissue was examined by western blot. Result: Compared with normal group

blood lipid

liver fat

free fatty acide(FFA)

insulin (INS)

insulin resistance index(HOMAR-IR)

malondialdehyde(MDA)

the SREBP-1c mRNA and protein expression was significantly increased in model group (P<0.05 or P<0.01);superoxide dismutase(SOD) content decreased significantly (P<0.01).Compared with the model group

the hepatic Steatosis degree was alleviated obviously in the Xiaozhi decoction-high-dose group. TC

AST

ALT

GGT content in serum

and TC

TG content in liver tissue were dramatically declined(P<0.05 or P<0.01). The SREBP-1c mRNA and protein in treatment groups was down-regulated (P<0.01)

especially in the Xiaozhi decoction-high-dose group

whose expression was much lower compared with model group(P<0.01). Conclusion: Xiaozhi decoction showed certain therapeutic effect on NAFLD

which might be linked to down-regulating SREBP-1c mRNA and protein expression.

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