HPLC同时测定猪胆汁水解产物中猪去氧胆酸、鹅去氧胆酸的含量

陈悦; 王静; 陈剑锋; 吕佳; 袁子民

doi:10.13422/j.cnki.syfjx.2014190061

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HPLC同时测定猪胆汁水解产物中猪去氧胆酸、鹅去氧胆酸的含量

化学与分析 | 更新时间：2024-01-04

- HPLC同时测定猪胆汁水解产物中猪去氧胆酸、鹅去氧胆酸的含量
- Determination of Contents of Hyodeoxycholic Acid and Chenodeoxycholic Acid in Hydrolysate of Fel Suillus by HPLC
- 中国实验方剂学杂志 2014年20卷第19期页码：61-63
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（81303225）
- DOI：10.13422/j.cnki.syfjx.2014190061
  中图分类号： R284.1
- 纸质出版日期：2014
- 稿件说明：
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陈悦, 王静, 陈剑锋, 等. HPLC同时测定猪胆汁水解产物中猪去氧胆酸、鹅去氧胆酸的含量[J]. 中国实验方剂学杂志, 2014,20(19):61-63.

CHEN Yue, WANG Jing, CHEN Jian-feng, et al. Determination of Contents of Hyodeoxycholic Acid and Chenodeoxycholic Acid in Hydrolysate of Fel Suillus by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(19): 61-63.
陈悦, 王静, 陈剑锋, 等. HPLC同时测定猪胆汁水解产物中猪去氧胆酸、鹅去氧胆酸的含量[J]. 中国实验方剂学杂志, 2014,20(19):61-63. DOI： 10.13422/j.cnki.syfjx.2014190061.

CHEN Yue, WANG Jing, CHEN Jian-feng, et al. Determination of Contents of Hyodeoxycholic Acid and Chenodeoxycholic Acid in Hydrolysate of Fel Suillus by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(19): 61-63. DOI： 10.13422/j.cnki.syfjx.2014190061.

摘要

目的：建立同时测定猪胆汁水解产物中猪去氧胆酸和鹅去氧胆酸含量的高效液相色谱方法。方法：采用C18色谱柱（4.6 mm×200 mm，5 μm），以乙腈-0.1%磷酸（45：55）为流动相，检测波长192 nm，流速1.0 mL·min-1，柱温35 ℃。结果：猪去氧胆酸在1.027～8.216 μg具有良好的线性关系（r=0.999 9），平均回收率99.0%（RSD 1.94%）。鹅去氧胆酸进样量0.963～7.704 μg具有良好的线性关系（r=0.999 0），平均回收率99.5%（RSD 1.76%）。结论：该方法灵敏、准确简便，适用于猪胆汁水解产物中猪去氧胆酸和鹅去氧胆酸的含量测定。

Abstract

Objective: The aim of this study was to establish a high-performance liquid chromatography(HPLC)method for the determination of both hyodeoxycholic acid and henodeoxycholic acid in the hydrolysate of Fel Suillus simultaneously. Method: The column named Diamonsil C18 (4.6 mm×200 mm

5 μm) was used for the chromatographic separation. Acetonitrile-0.1%phosphoric acid(45:55)was used as the mobile phase. The flow-rate was set at 1.0 mL·min-1. The detection wavelength was set at 192 nm. The column temperature was maintained at 35 ℃ and the injection volume was 10 μL. Result: Both hyodeoxycholic acid and chenodeoxycholic acid showed good linear relationship in the range of 1.027-8.216 μg and 0.963-7.704 μg

with the correlation coefficients of 0.999 9 and 0.999 0

respectively. The average recovery rates were 99.0% (RSD 1.94%) and 99.5%(RSD 1.76%). Conclusion: The method is accurate

suitable

simple

and can be used for the simultaneous determination of hyodeoxycholic acid and chenodeoxycholic acid in the hydrolysate of Fel Suillus by HPLC.

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