Objective: The purpose of the study was to establish a method for the determination of the content of quercetin and total flavonoids in Trichosanthis Pericarpium

and to determine the content of quercetin and total flavonoids in Trichosanthis Pericarpium from different areas. Method: Diverse concentrations of ethanol and methanol were chosen as the extraction solvents for quercetin while it was extracted by immersion extraction

ultrasonic extraction

and refluxing process

then the content was determined by HPLC. The content of total flavonoids was determined using ultraviolet spectrophotometry after it was extracted by ethanol (75%). Result: Refluxing process with methanol proved to be the optimal extraction method for quercetin. The Pericarpium trichosanthis from Jiangsu province showed higher content of quercetin

additionally

the content of total flavonoids in Trichosanthis Pericarpium from Anhui province was much more than other areas. Conclusion: The method established to determine the content of quercetin and total flavonoids in Trichosanthis Pericarpium in this study exhibits good reproducibility and accuracy. The contents of quercetin and total flavonoids in Trichosanthis Pericarpium were significantly different in different areas.