分散液液微萃取-高效液相色谱法测定金银花中拟除虫菊酯类农药残留

周卿; 周旭美

doi:10.13422/j.cnki.syfjx.2014200070

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分散液液微萃取-高效液相色谱法测定金银花中拟除虫菊酯类农药残留

化学与分析 | 更新时间：2024-01-04

- 分散液液微萃取-高效液相色谱法测定金银花中拟除虫菊酯类农药残留
- Determination of Pyrethroid Pesticide Residues in Lonicerae Janponicae Flos by Disperse Liquid-liquid Microextraction Coupled with HPLC
- 中国实验方剂学杂志 2014年20卷第20期页码：70-73
- 作者机构：
- 作者简介：
- 基金信息：
  
  贵州省中医药管理局项目（QZYY2012-D406）
- DOI：10.13422/j.cnki.syfjx.2014200070
  中图分类号： R284.1
- 纸质出版日期：2014
- 稿件说明：
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周卿, 周旭美. 分散液液微萃取-高效液相色谱法测定金银花中拟除虫菊酯类农药残留[J]. 中国实验方剂学杂志, 2014,20(20):70-73.

ZHOU Qing, ZHOU Xu-mei. Determination of Pyrethroid Pesticide Residues in Lonicerae Janponicae Flos by Disperse Liquid-liquid Microextraction Coupled with HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(20): 70-73.
周卿, 周旭美. 分散液液微萃取-高效液相色谱法测定金银花中拟除虫菊酯类农药残留[J]. 中国实验方剂学杂志, 2014,20(20):70-73. DOI： 10.13422/j.cnki.syfjx.2014200070.

ZHOU Qing, ZHOU Xu-mei. Determination of Pyrethroid Pesticide Residues in Lonicerae Janponicae Flos by Disperse Liquid-liquid Microextraction Coupled with HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(20): 70-73. DOI： 10.13422/j.cnki.syfjx.2014200070.

摘要

目的：建立分散液液微萃取-高效液相色谱法测定金银花中氟氰戊菊酯、氰戊菊酯、高效氟氯氰菊酯、氯菊酯、联苯菊酯5种拟除虫菊酯类农药残留的方法。方法：以乙腈为分散剂，三氯甲烷为萃取剂富集金银花中5种菊酯类农药，并优化影响富集效率的因素；采用HPLC测定，Agilent ZORBAX XDB-C18色谱柱（4.6 mm×250 mm，5 μm），流动相乙腈-水梯度洗脱，检测波长230 nm，流速1 mL ·min-1。结果：氟氰戊菊酯、氰戊菊酯、高效氟氯氰菊酯、氯菊酯、联苯菊酯在0.05~5.0 mg ·L-1线性关系良好（r>0.999 0），富集倍数在18.6~29.7，高、中、低3种浓度平均加样回收率在72.1%~110.4%。结论：该方法操作简便、准确性好，灵敏度高，可满足菊酯类农药残留分析的要求。

Abstract

Objective: This study was aimed to establish disperse liquid-liquid microextraction coupled with high performance liquid chromatography(DLLME-HPLC) method for determination of five pyrethroid pesticides (flucythrinate

fenvalerate

lambda-cyhalothrin

permethrin

bifenthrin) in Lonicerae Janponicae Flos. Method: The five pyrethroid pesticides in Lonicerae Janponicae Flos were extracted by trichlormethane

with acetonitrile as dispersant

then analysed by HPLC.Some important parameters that influenced the extraction efficiency were optimized. The five pesticides were separated by an Agilent ZORBAX XDB-C18(4.6 mm×250 mm

5 μm) column in the wavelength at 230 nm. The mobile phase was acetonitrile-H2O with gradient elution and the flow rate was 1.0 mL·min-1. Result: Flucythrinate

fenvalerate

lambda-cyhalothrin

permethrin and bifenthrin showed good linear relation within range of 0.05-5.0 mg·L-1 (r>0.999 0). The enrichment factors ranged from 18.6 to 29.7.The average recovery of high

medium and low concentration ranged from 72.1% to 110.4%. Conclusion: The method is accurate and sensitive

simple and fast

and can meet the requirement of pyrethroid pesticedes residue analysis in Lonicerae Janponicae Flos.

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