二至丸不同极性部位对抗大鼠酒精性肝损伤的作用及机制

聂映; 姚卫峰

doi:10.13422/j.cnki.syfjx.2014200145

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二至丸不同极性部位对抗大鼠酒精性肝损伤的作用及机制

药理 | 更新时间：2024-01-04

- 二至丸不同极性部位对抗大鼠酒精性肝损伤的作用及机制
- Protective Effect of Different Polar Fraction of Erzhi Pill on Alcoholic Liver Injury
- 中国实验方剂学杂志 2014年20卷第20期页码：145-149
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（81001599）;教育部博士点新教师基金项目（20103237120010）
- DOI：10.13422/j.cnki.syfjx.2014200145
  中图分类号： R285.5
- 纸质出版日期：2014
- 稿件说明：
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聂映, 姚卫峰. 二至丸不同极性部位对抗大鼠酒精性肝损伤的作用及机制[J]. 中国实验方剂学杂志, 2014,20(20):145-149.

NIE Ying, YAO Wei-feng. Protective Effect of Different Polar Fraction of Erzhi Pill on Alcoholic Liver Injury[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(20): 145-149.
聂映, 姚卫峰. 二至丸不同极性部位对抗大鼠酒精性肝损伤的作用及机制[J]. 中国实验方剂学杂志, 2014,20(20):145-149. DOI： 10.13422/j.cnki.syfjx.2014200145.

NIE Ying, YAO Wei-feng. Protective Effect of Different Polar Fraction of Erzhi Pill on Alcoholic Liver Injury[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(20): 145-149. DOI： 10.13422/j.cnki.syfjx.2014200145.

摘要

目的：探讨二至丸不同提取部位对抗大鼠酒精性肝损伤的作用及机制。方法：将二至丸水提液依次用不同溶剂萃取，经干燥后得到石油醚、乙酸乙酯、正丁醇和萃余液4个部位浸膏，加水溶解配制溶液。取70只雄性Wistar大鼠随机分为7组，空白对照组，模型组，阳性药硫普罗宁肠溶片组（60 mg ·kg-1），二至丸水提液石油醚、乙酸乙酯、正丁醇萃取部位和萃余液4个部位组（15.12 g ·kg-1），造模同时连续ig给药28 d。以乙醇灌胃法连续28 d建立酒精性肝损伤模型，末次给药24 h后，所有大鼠采用颈动脉采血，分离血清检测血清丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、乙醇脱氢酶（ADH）、甘油三酯（TG）、血清内毒素、肿瘤坏死因子α（TNF-α）、干扰素γ（IFN-γ）、白细胞介素18（IL-18）的活性和肝匀浆超氧化物歧化酶（SOD）、丙二醛（MDA），观察肝脏病理形态学改变。结果：与空白对照组相比，模型组血清ALT，AST，ADH，TG，血清内毒素，TNF-α，IFN-γ及IL-18活性明显升高，有显著性差异（P<0.01），表明模型制备成功；各给药组ALT，AST，ADH，TG指标的升高被显著抑制，与模型组相比有差异（P<0.01或P<0.05）。与空白对照组相比，模型组肝匀浆中MDA含量明显升高，SOD活性下降（P<0.01），与模型组相比硫普罗宁、二至丸水提液石油醚部位组和乙酸乙酯部位组MDA的升高被显著抑制（P<0.01或P<0.05）；与模型组相比，硫普罗宁、乙酸乙酯组、正丁醇组和萃余液组SOD的下降被明显抑制（P<0.01或P<0.05）。通过病理学切片观察，各给药组均一定程度能显著改善肝组织的病理变化。结论：二至丸乙酸乙酯部位对抗大鼠酒精性肝损伤作用最显著，其作用机制可能与内毒素-CD14/TLR4的抑制作用有关。

Abstract

Objective: To investigate the protective effect of different polar fraction of Erzhi pill on acute liver injure of rats induced by alcohol and its mechanism. Method: Water decoction of Erzhi pill was extracted in order by different solvents. After drying

petroleum leve

acetoacetate and n-butanol fractions were obtained

and dissolved by water. Seventy male Wistar rats were randomly divided into seven groups. The model of alcoholic liver injury in rats was established by giving alcohol ig per day for 28 days. The serum alanine aminotransferase (ALT)

aspartate aminotransferase (AST)

alcohol dehydrogenase (ADH) and triglycerides (TG)

serum endotoxin

tumor necrosis factor-α (TNF-α)

interferon-γ (IFN-γ)

interleukin-18 (IL-18) were assayed. And hepatic superoxide dismutase(SOD)

malondialdehyde(MDA) were measured respectively. Hepatic histopathology was observed by microscope. Result: Combined with the control group

serum levels of ALT

AST

ADH

serum endotoxin

TNF-α

IFN-γ and IL-18 of the model group increased significantly (P<0.01)

indicating that the model was established successfully;and the increasing levels of ALT

AST

ADH

serum endotoxin

TNF-α

IFN-γ and IL-18 of all the treatment groups were inhibited (compared with model group

P<0.05 or P<0.01).Combined with the control group

hepatic levels of MDA and SOD in model group increased or decreased significantly (P<0.01)

the increasing levels of MDA in the tiopronin group

petroleum leve and acetoacetate groups were inhibited (compared with model group

P<0.05 or P<0.01). The decreasing levels of SOD in the tiopronin group

acetoacetate

n-butanol and raffinate groups were inhibited (compared with model group

P<0.05 or P<0.01). The histopathological analysis suggested that all of the treatment groups could significantly ameliorate pathological changes of hepatocytes. Conclusion: Ethyl acetate fraction of Erzhi pill has the protective effect on liver injury of rats induced by alcohol

the mechanism might be related to endotoxin-CD14/TLR4.

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