HAO Peng-fei, ZHANG Li-fang, ZHANG Chao-yun, et al. Comparative Analysis GC-MS of Artemisiae Argyi Folium Volatile Oil Extracted by SPME and SFE[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(23): 68-71.
HAO Peng-fei, ZHANG Li-fang, ZHANG Chao-yun, et al. Comparative Analysis GC-MS of Artemisiae Argyi Folium Volatile Oil Extracted by SPME and SFE[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(23): 68-71. DOI: 10.13422/j.cnki.syfjx.2014230068.
Objective: The aim of this study was to analyze the ingredients
and to identify the main ingredients and content of the volatile oil
which extracted from Nanyang Artemisiae Argyi Folium volatile oil by two different solid-phase microextraction (SPME) and supercritical fluid extraction (SFE). Method: The volatile oil was extracted separately from Artemisiae Argyi Folium by SPME and SFE. Then GG-MS was used to analyze it. Result: Thirty-eight components were isolated by SPEM
accounting for 95.17% of the total content
Thirty-five components were with 35 identified by comparing with the mass spectrometry database
while 26 components were isolated by SFE
accounting for 95.26% of the total content
with 25 identified by comparing with the mass spectrometry database. The content of each component varied
with eucaylptole as the most (13.75% and 18.26% respectively). The following components isolated from SPME were including borneol (11.58%)
isoartemisia ketone (7.15%) and esym-dimethylurea (5.12%);the following components from SFE included isoartemisia ketone (12.15%)
borneol (11.38%) and trans-ocimene (7.03%). Conclusion: The result verified the superiority of the SPME method
though the components of the volatile oil extracted by two different ways are obviously different.