HPLC测定3种葛根素注射液中葛根素含量

李昌勤; 李丹; 王培卿; 张丹; 康文艺; 程力

doi:10.13422/j.cnki.syfjx.2014240060

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HPLC测定3种葛根素注射液中葛根素含量

化学与分析 | 更新时间：2024-01-04

- HPLC测定3种葛根素注射液中葛根素含量
- Determintion for Content of Puerarin in Three Kinds of Puerarin Injections by HPLC Method
- 中国实验方剂学杂志 2014年20卷第24期页码：60-63
- 作者机构：
- 作者简介：
- 基金信息：
  
  河南省科技厅重点攻关项目(122102310272);河南大学研究生教育综合改革项目(Y141108)
- DOI：10.13422/j.cnki.syfjx.2014240060
  中图分类号： O657.72;R284.1
- 纸质出版日期：2014
- 稿件说明：
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李昌勤, 李丹, 王培卿, 等. HPLC测定3种葛根素注射液中葛根素含量[J]. 中国实验方剂学杂志, 2014,20(24):60-63.

LI Chang-qin, LI Dan, WANG Pei-qing, et al. Determintion for Content of Puerarin in Three Kinds of Puerarin Injections by HPLC Method[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(24): 60-63.
李昌勤, 李丹, 王培卿, 等. HPLC测定3种葛根素注射液中葛根素含量[J]. 中国实验方剂学杂志, 2014,20(24):60-63. DOI： 10.13422/j.cnki.syfjx.2014240060.

LI Chang-qin, LI Dan, WANG Pei-qing, et al. Determintion for Content of Puerarin in Three Kinds of Puerarin Injections by HPLC Method[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(24): 60-63. DOI： 10.13422/j.cnki.syfjx.2014240060.

摘要

目的: 应用高效液相色谱法同时测定葛根素注射液、葛根素葡萄糖注射液和葛根素氯化钠注射液中葛根素的含量. 方法: 采用Merk

Purospher STAR RP-C18色谱柱(4.6 mm×250 mm

5 μm)

流动相甲醇-1%冰乙酸水溶液(25:75)

流速1.0 mL·min-1

柱温29 ℃

检测波长250 nm

进样量10 μL. 结果: 对照品葛根素在0.192~1.152 μg呈良好曲线关系

线性回归方程Y=468 374X+3 105.25(r=0.999 8)

平均加样回收率为99.81%

RSD 0.42%. 结论: 3种注射液中的葛根素均可在选定的条件下得到较好的分离

线性关系良好.该方法简单快速、合理可行

可为葛根素注射液的质量控制提供科学依据.

Abstract

Objective: An HPLC method was established to determine the content of pueratin in three types of injection. Method: Separation was achieved by a Merk

Purospher STAR RP-C18 column used at 29 ℃

and mobile phase was methanol and 1% acetic acid

water with the flow rate of 1.0 mL·min-1

detection wavelength of 250 nm. Result: The puerarin was separated with good linearity in the sample size range of 0.192-1.152 μg. The average recovery was 99.81%

with RSD value of 0.42%. Conclusion: The puerarin in three types of puerarin injection could be well separated under the selected conditions

showing good linear relationship. The method was simple and quick

feasible and could provide scientific basis for the quality control of puerarin injection.

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