贵州苗族药酢浆草不同溶剂提取物的药效比较及HPLC测定

崔珺; 刘星星; 李月婷; 李靖; 何彬; 刘亭; 王永林; 何迅

doi:10.13422/j.cnki.syfjx.2015020053

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贵州苗族药酢浆草不同溶剂提取物的药效比较及HPLC测定

化学与分析 | 更新时间：2024-01-04

- 贵州苗族药酢浆草不同溶剂提取物的药效比较及HPLC测定
- Comparison of Effects and HPLC Detection on Different Extracts of Miao Medicine in Guizhou Province
- 中国实验方剂学杂志 2015年21卷第2期页码：53-56
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家科技支撑计划项目(2013BAI11B01);贵州省科学技术基金项目(黔科合J字[2011]2291号);贵州省中药现代化专项项目(黔科合中药字[2013]5062号,黔科合重G字[2013] 4001)
- DOI：10.13422/j.cnki.syfjx.2015020053
  中图分类号： R29
- 纸质出版日期：2015
- 稿件说明：
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崔珺, 刘星星, 李月婷, 等. 贵州苗族药酢浆草不同溶剂提取物的药效比较及HPLC测定[J]. 中国实验方剂学杂志, 2015,21(2):53-56.

CUI Jun, LIU Xing-xing, LI Yue-ting, et al. Comparison of Effects and HPLC Detection on Different Extracts of Miao Medicine in Guizhou Province[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(2): 53-56.
崔珺, 刘星星, 李月婷, 等. 贵州苗族药酢浆草不同溶剂提取物的药效比较及HPLC测定[J]. 中国实验方剂学杂志, 2015,21(2):53-56. DOI： 10.13422/j.cnki.syfjx.2015020053.

CUI Jun, LIU Xing-xing, LI Yue-ting, et al. Comparison of Effects and HPLC Detection on Different Extracts of Miao Medicine in Guizhou Province[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(2): 53-56. DOI： 10.13422/j.cnki.syfjx.2015020053.

摘要

目的: 研究酢浆草70%乙醇和水提取物的急性毒性、抗炎和镇痛作用

分析HPLC图中峰信息量。方法: 采用急性毒性最大耐受量试验评价酢浆草70%乙醇和水提取物的急性毒性;冰乙酸致腹腔毛细血管通透性试验考察抗炎作用

冰醋酸致扭体反应考察镇痛作用;使用HPLC对不同溶剂提取物进行分析。结果: 在35～1 120 mg ·kg-1的剂量范围内各组小鼠活动正常

未见死亡

MTD大于1 120 mg ·kg-1;与水提取物比较

70%乙醇提取物能更好地抑制冰乙酸引起的毛细血管通透性增高及扭体反应

HPLC峰信息更丰富

共有峰的提取率更高。结论: 酢浆草70%乙醇和水提取物的致死剂量较高

毒性较小

70%乙醇提取物的抗炎镇痛作用优于水提取物

并且能够更充分的富集有效成分。

Abstract

Objective: To study acute toxicity

anti-inflammatory and anti-nociceptive effects of 70% ethanol extract (E70%EtOH) and water extract (Ewater) of Oxalis corniculata

HPLC was used to analyze figure information and determine the best extrat. Method: The maximum dosage test was used to evaluate safety of E70%EtOH and Ewater. Permeability increasion of celiac capillary induced by glacial acetic acid in mice was performed to investigate the anti-inflammatory effect

writhing test induced by glacial acetic acid in mice was used to observe the anti-nociceptive activity. Analyze different extracts by HPLC. Result: E70%EtOHand Ewater didn't show toxicity in the range of 35 to 1 120 mg · kg-1

MTD>1 120 mg · kg-1. Compare with Ewater

E70%EtOH could remarkable inhibit the permeability increasion of celiac capillary and the writhing reaction in mice

HPLC peak information is richer and the common peak ratio is higher than that of Ewater. Conclusion: Lethal dose of E70%EtOH and Ewater may be very high and toxicity of E70%EtOH and Ewater may be very low. The effects of E70%EtOH is better than that of Ewater

E70%EtOH can more fully the enrichment of active component.

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