Objective: To investigate the chemical constituents of n-butanol extract of Ginseng medicinal fungal substance

which was to clarify the basis of its efficacy substance. Method: Thin-layer chromatography

column chromatography on silica gel

Sephadex LH-20 gel chromatography

semi-preparative liquid chromatography were used for the isolation and purification of the compounds and their structures were determined by comparison of their chromatographic and spectral data with those of the authentic samples and those reported in the literature. Result: Five compounds from n-butanol extract of ginseng medicinal fungal substance were identified as 20(S)-ginsenosides-Rg2 (1)

20(R)-Ginsenosides-Rg2 (2)

Ginsenosides-Rg1 (3)

20(S)-Ginsenosides-Rf (4)

ginsenosides-Rb1 (5). Conclusion: All compounds are isolated from ginseng medicinal fungal substance for the first time.