Objective: To optimize separation and purification technology conditions of evodiamine from ethanol extract of Euodiae Fructus by macroporous resin. Method: With adsorption volume and elution rate of evodiamine as indexes

five kinds(AB-8

S-8

NKA-9

HPD-100

D-101) of macroporous resins were selected by static adsorption and desorption test.Sample solution concentration

sample volume

sample flow rate

eluent type

eluent amount and other parameters were investigated by single factor tests. Result: D-101 macroporous resin had the best separation and purification capacity for evodiamine in following technology conditions:sample concentration of 3 g · L-1

sample volume of 4 BV

sample flow rate of 1 BV · h-1

removed water-soluble impurities with 5 BV of water

eluted with 5 BV of 80% ethanol

eluting velocity of 2 BV · h-1.Elution rate of evodiamine was 92.81%

its purity increased from 1.29% to 26.77%. Conclusion: D-101 macroporous resin is suitable for purifying evodiamine from Euodiae Fructus.This optimized method is stable and feasible

it can provide a reference for development and utilization of evodiamine.