麸炒前后苍术HPLC指纹图谱

常晓文; 刘玉强; 才谦

doi:10.13422/j.cnki.syfjx.2015040040

您当前的位置：

首页 >

文章列表页 >

麸炒前后苍术HPLC指纹图谱

化学与分析 | 更新时间：2024-01-04

- 麸炒前后苍术HPLC指纹图谱
- Fingerprints of Raw and Processed Atractylodis Rhizoma by HPLC
- 中国实验方剂学杂志 2015年21卷第4期页码：40-43
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目(81202919);国家中医药管理局中医药行业科研专项(20110700712)
- DOI：10.13422/j.cnki.syfjx.2015040040
  中图分类号： R283.1;R284.1
- 纸质出版日期：2015
- 稿件说明：
移动端阅览
常晓文, 刘玉强, 才谦. 麸炒前后苍术HPLC指纹图谱[J]. 中国实验方剂学杂志, 2015,21(4):40-43.

CHANG Xiao-wen, LIU Yu-qiang, CAI Qian. Fingerprints of Raw and Processed Atractylodis Rhizoma by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(4): 40-43.
常晓文, 刘玉强, 才谦. 麸炒前后苍术HPLC指纹图谱[J]. 中国实验方剂学杂志, 2015,21(4):40-43. DOI： 10.13422/j.cnki.syfjx.2015040040.

CHANG Xiao-wen, LIU Yu-qiang, CAI Qian. Fingerprints of Raw and Processed Atractylodis Rhizoma by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(4): 40-43. DOI： 10.13422/j.cnki.syfjx.2015040040.

摘要

目的: 通过建立生苍术和麸炒苍术的高效液相指纹图谱

比较苍术麸炒前后化学成分的差异

为麸炒苍术炮制机制的研究提供科学依据。方法: 采用Agilent 1100 series 高效液相色谱仪

Promosil C18色谱柱(4.6 mm×250 mm

5 μm)

以乙腈-0.1％磷酸水为流动相

梯度洗脱

流速1.0 mL ·min-1

检测波长270 nm。结果: 分别建立了生苍术和麸炒苍术的高效液相指纹图谱

各标定18个共有峰

相似度均大于0.90。麸炒后苍术中包括苍术素醇

(4E

12E)-十四癸三烯-8

10-二炔-1

3-二乙酸酯和苍术素在内的多个色谱峰的峰面积值下降。结论: 运用高效液相法可对苍术麸炒前后化学成分进行分析

实验方法准确可靠。

Abstract

Objective: To study the differences of chemical components in raw and processed Atractylodis Rhizoma and to provide a scientific basis and evidence for clarifying the mechanism of processing. Method: Agilent C18column(4.6 mm×250 mm

5 μm)was used with the mobile phase of acetonitrile and water

the flow rate was 1 mL · min-1

the detection wavelength was set at 270 nm. Result: The HPLC fingerprints of raw and processed Atractylodis Rhizoma were established and 18 common peaks were detected

the similarity of 10 batches raw and processed Atractylodis Rhizoma were over 0.9

the peak areas of multi-components including atractylodin

atractylodes lancea and (4E

12E)-tetradecatriene-8

10-diyne-1

3-diyl diacetate were decreased after processing. Conclusion: HPLC fingerprint has been established to analyze the chemical components in raw and processed Atractylodis Rhizoma

the method is accurate and reliable.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

四物微丸高效液相法指纹图谱的研究

通窍活血汤的液相指纹图谱研究

不同主产地黑顺片的指纹图谱研究

苍术炒焦前后GC指纹图谱的比较

假茼蒿HPLC指纹图谱