WANG Dan, FAN Zuo-wang, PANG Yu-xin, et al. Effect of Exogenous Magnesium on Biomass and Contents of Effective Constituents of in Slow Growth Period of Winter[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(4): 75-79.
WANG Dan, FAN Zuo-wang, PANG Yu-xin, et al. Effect of Exogenous Magnesium on Biomass and Contents of Effective Constituents of in Slow Growth Period of Winter[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(4): 75-79. DOI: 10.13422/j.cnki.syfjx.2015040075.
显著增加了艾纳香叶、茎和根的生物量。10 g ·L-1镁处理的艾纳香叶片和茎生物量分别为226.47
140.40 g
依次为空白组(CK)叶片(22.45 g)和茎(26.57 g)生物量的10.09
5.28倍;15 g ·L-1镁处理下的艾纳香叶片和茎生物量分别为244.88
146.02 g。外源镁对艾纳香不同部位中总黄酮和l-龙脑质量分数提高无促进作用或影响不显著
但增加了二者质量的积累
10
15 g ·L-1镁处理下的l-龙脑质量分别是CK的8.5
10.0倍。 结论: 在冬季生长迟缓期施加镁
可促进艾纳香的生长和生物量积累
显著提高其总黄酮和l-龙脑的质量。
Abstract
Objective: To investigate effect of exogenous magnesium(Mg) on biomass and contents of effective constituents of Blumea balsamifera in slow growth period of winter. Method: One-year-old B. balsamifera seedlings were applied with MgSO4 · 7H2O which supplied Mg in slow growth period of winter three times.Height
ground diameter
length of leaf
width of leaf and biomass were measured.UV was adopted to determine the content of total flavonoids in different parts of B. balsamifera with detection wavelength of 500 nm.The content of l-bornel in leaves of B. balsamifera were determined by GC
initial temperature was 80 ℃ for 2 min
then warmed to 100 ℃ by 5 ℃ · min-1 at 4 min
and warmed to 200 ℃ by 10 ℃ · min-1 at 10 min.Temperature of injection port was 220 ℃ and FID detector temperature was 240 ℃
injection volume was 0.6 μL and split ratio was 9: 1. Result: Exogenous Mg significantly enhanced growth indexes of this plant
especially of ground diameter
length and width of leaf.As a result
exogenous Mg increased significantly biomasses of leaves
stems and roots
respectively.Biomasses of leaves and stems under 10 g · L-1 Mg treatment were 226.47 g and 140.40 g
which were 10.09 and 5.28 times compared with the control black treatment(CK)
respectively.Biomasses of leaves and stems under 15 g · L-1 Mg treatment were 244.88 g and 146.02 g.However
exogenous Mg could not enhance or affect indistinctively the mass fraction of total flavonoids and l-borneol
but it increased qualities of total flavonoids and l-borneol significantly.Qualities of l-borneol under 10
15 g · L-1 Mg treatments were 8.5 and 10.0 times by comparing with CK
respectively. Conclusion: Exogenous Mg can promote plant growth and biomass accumulation
significantly increase qualities of total flavonoids and l-borneol in B. balsamifera in slow growth period of winter.