HPLC-DAD测定茵陈蒿汤中儿茶素、绿原酸、西红花苷Ⅰ、西红花苷Ⅱ的含量

候金燕; 罗琳; 窦志华; 冯艳; 陆群; 宣东平; 陈霞; 顾薇

doi:10.13422/j.cnki.syfjx.2015060048

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HPLC-DAD测定茵陈蒿汤中儿茶素、绿原酸、西红花苷Ⅰ、西红花苷Ⅱ的含量

化学与分析 | 更新时间：2024-01-04

- HPLC-DAD测定茵陈蒿汤中儿茶素、绿原酸、西红花苷Ⅰ、西红花苷Ⅱ的含量
- Determination of Catechin, Chlorogenic Acid, Crocin-Ⅰ, Crocin-Ⅱ in Yinchenhao Decoction by HPLC-DAD
- 中国实验方剂学杂志 2015年21卷第6期页码：48-51
- 作者机构：
- 作者简介：
- 基金信息：
  
  江苏省中医药局科技项目(HZ07071);江苏省高校自然科学基金项目(08KJB360009)
- DOI：10.13422/j.cnki.syfjx.2015060048
  中图分类号： O657.72;R286.0
- 纸质出版日期：2015
- 稿件说明：
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候金燕, 罗琳, 窦志华, 等. HPLC-DAD测定茵陈蒿汤中儿茶素、绿原酸、西红花苷Ⅰ、西红花苷Ⅱ的含量[J]. 中国实验方剂学杂志, 2015,21(6):48-51.

HOU Jin-yan, LUO Lin, DOU Zhi-hua, et al. Determination of Catechin, Chlorogenic Acid, Crocin-Ⅰ, Crocin-Ⅱ in Yinchenhao Decoction by HPLC-DAD[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(6): 48-51.
候金燕, 罗琳, 窦志华, 等. HPLC-DAD测定茵陈蒿汤中儿茶素、绿原酸、西红花苷Ⅰ、西红花苷Ⅱ的含量[J]. 中国实验方剂学杂志, 2015,21(6):48-51. DOI： 10.13422/j.cnki.syfjx.2015060048.

HOU Jin-yan, LUO Lin, DOU Zhi-hua, et al. Determination of Catechin, Chlorogenic Acid, Crocin-Ⅰ, Crocin-Ⅱ in Yinchenhao Decoction by HPLC-DAD[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(6): 48-51. DOI： 10.13422/j.cnki.syfjx.2015060048.

摘要

目的: 建立HPLC-DAD测定茵陈蒿汤中儿茶素、绿原酸、西红花苷Ⅰ、西红花苷Ⅱ含量的方法。方法: 采用Symmetry C18色谱柱(4.6 mm×250 mm

5 μm)

流动相乙腈-0.1%磷酸水梯度洗脱

流速1.0 mL ·min-1

柱温30 ℃

儿茶素检测波长202 nm

绿原酸检测波长330 nm

西红花苷Ⅰ

Ⅱ检测波长440 nm。结果: 儿茶素、绿原酸、西红花苷Ⅰ

Ⅱ分别在0.18~0.9(r=0.999 8)

0.062 4~0.312(r=0.999 9)

0.441~2.205(r=0.999 9)

0.023 52~0.117 6 μg(r=0.999 9)与峰面积呈良好线性关系;加样回收率分别为98.56%(RSD 1.8%)

99.07%(RSD 1.8%)

97.41%(RSD 1.9%)和98.46%(RSD 1.9%)。结论: 该法简便、准确、专属性强

可用于茵陈蒿汤中4种成分的含量测定。

Abstract

Objective: To establish a method for simultaneous determination of catechin

chlorogenic acid

crocin-Ⅰ

and crocin-Ⅱ in Yinchenhao decoction by HPLC-DAD. Method: The Symmetry C18 column (4.6 mm×250 mm

5 μm) was used

the mobile phase was acetonitrile-0.1% phosphoric acid solution with gradient elution at a flow rate of 1.0 mL · min-1. The column temperature was kept at 30 ℃ and the wavelength was set at 202 nm for catechin

330 nm for chlorogenic acid

and 440 nm for crocin-Ⅰand crocin-Ⅱ

respectively. Result: The linear range of catechin

chlorogenic acid

crocin-Ⅰ

and crocin-Ⅱ were 0.18-0.90 (r=0.999 8)

0.020 8-0.374 4 (r=0.999 9)

0.147-2.646 (r=0.999 9) and 0.007 84-0.141 12 (r=0.999 9) μg

respectively. The mean recovery of these 4 components were 98.56% (RSD 1.8%)

99.07% (RSD 1.8%)

97.41% (RSD 1.9%)

and 98.46% (RSD 1.9%)

respectively. Conclusion: This method is simple

accurate and specific

and can be used for the determination of 4 constituents in Yinchenhao decoction.

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