HPLC同时测定脉平片中白果内酯、银杏内酯A和银杏内酯B、银杏内酯C的含量

倪琳; 张秀娟; 高进贤; 杨锡

doi:10.13422/j.cnki.syfjx.2015060066

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HPLC同时测定脉平片中白果内酯、银杏内酯A和银杏内酯B、银杏内酯C的含量

化学与分析 | 更新时间：2024-01-04

- HPLC同时测定脉平片中白果内酯、银杏内酯A和银杏内酯B、银杏内酯C的含量
- Determination of Bilobalide, Ginkgolide A, Ginkgolicle B and Ginkgolicle C in Maiping Tablet by HPLC
- 中国实验方剂学杂志 2015年21卷第6期页码：66-68
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2015060066
  中图分类号： O657.72;R286.0
- 纸质出版日期：2015
- 稿件说明：
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倪琳, 张秀娟, 高进贤, 等. HPLC同时测定脉平片中白果内酯、银杏内酯A和银杏内酯B、银杏内酯C的含量[J]. 中国实验方剂学杂志, 2015,21(6):66-68.

NI Lin, ZHANG Xiu-juan, GAO Jin-xian, et al. Determination of Bilobalide, Ginkgolide A, Ginkgolicle B and Ginkgolicle C in Maiping Tablet by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(6): 66-68.
倪琳, 张秀娟, 高进贤, 等. HPLC同时测定脉平片中白果内酯、银杏内酯A和银杏内酯B、银杏内酯C的含量[J]. 中国实验方剂学杂志, 2015,21(6):66-68. DOI： 10.13422/j.cnki.syfjx.2015060066.

NI Lin, ZHANG Xiu-juan, GAO Jin-xian, et al. Determination of Bilobalide, Ginkgolide A, Ginkgolicle B and Ginkgolicle C in Maiping Tablet by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(6): 66-68. DOI： 10.13422/j.cnki.syfjx.2015060066.

摘要

目的: 建立脉平片中白果内酯、银杏内酯A、银杏内酯B和银杏内酯C的含量测定方法。方法: 采用HPLC对制剂中银杏叶提取物的主要成分白果内酯、银杏内酯A、银杏内酯B和银杏内酯C进行定量分析。CAPCELL PAK C18色谱柱(4.6 mm×250 mm 5 μm);流动相四氢呋喃-乙腈-水(12:10:78)

蒸发光散射检测器

柱温30 ℃

流速1.0 mL ·min-1。结果: 银杏内酯C在1.11～6.66 μg

白果内酯在2.232～13.392 μg

银杏内酯A在1.912～11.472 μg

银杏内酯B在1.502～9.004 μg均呈良好的线性关系

平均回收率分别为96.36%

97.18%

97.71%

96.57%;RSD分别为1.0%

1.4%

2.1%

1.4%。结论: 方法准确可靠

专属性强

结果稳定

重复性好

可有效控制制剂质量。

Abstract

Objective: To establish an method for determination bilobalide

ginkgolide A

ginkgolicle B and ginkgolicle C in Maiping tablet. Method: The bilobalide

ginkgolide A

ginkgolicle B and ginkgolicle C in Maiping tablet was determined by HPLC. CAPCELL PAK C18(4.6 mm×250 mm 5 μm)column was used. The modbile phase was tetrahydrofuran- acetonitrile-water(12:10:78)with the flow rate of 1.0 mL ·min-1.The column temperature was 30 ℃ and evaporative light-scatting mass detector. Result: The ginkgolicle C

bilobalide

ginkgolide A and ginkgolicle B were linear at the range of 1.11-6.66

2.232-13.392

1.912-11.472

1.502-9.004 μg

respectively. The average recovery was 96.36%

97.18%

97.71%

96.57%and RSD was 1.0%

1.4%

2.1%

1.4%(n=6). Conclusion: The established method is accurate

reliable and specific. The results are stable with good reproducibility. The quality of Maiping tablet can be controlled by the method.

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