HPLC测定中药鸦胆子油中油酸和亚油酸

张悦; 张溪桐; 张彤; 闫玉娇; 王冰

doi:10.13422/j.cnki.syfjx.2015070051

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HPLC测定中药鸦胆子油中油酸和亚油酸

化学与分析 | 更新时间：2024-09-23

- HPLC测定中药鸦胆子油中油酸和亚油酸
- Determination of Oleic Acid and Linoleic Acid in Bruceae Fructus Oil by HPLC-UV
- 中国实验方剂学杂志 2015年21卷第7期页码：51-53
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目(81303233);上海市卫生局项目(20124074);上海市卫生局青年科研项目(20124Y002)
- DOI：10.13422/j.cnki.syfjx.2015070051
  中图分类号： O657.72;R284.1
- 纸质出版日期：2015
- 稿件说明：
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张悦, 张溪桐, 张彤, 等. HPLC测定中药鸦胆子油中油酸和亚油酸[J]. 中国实验方剂学杂志, 2015,21(7):51-53.

ZHANG Yue, ZHANG Xi-tong, ZHANG Tong, et al. Determination of Oleic Acid and Linoleic Acid in Bruceae Fructus Oil by HPLC-UV[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(7): 51-53.
张悦, 张溪桐, 张彤, 等. HPLC测定中药鸦胆子油中油酸和亚油酸[J]. 中国实验方剂学杂志, 2015,21(7):51-53. DOI： 10.13422/j.cnki.syfjx.2015070051.

ZHANG Yue, ZHANG Xi-tong, ZHANG Tong, et al. Determination of Oleic Acid and Linoleic Acid in Bruceae Fructus Oil by HPLC-UV[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(7): 51-53. DOI： 10.13422/j.cnki.syfjx.2015070051.

摘要

目的: 建立高效液相测定中药鸦胆子油中油酸和亚油酸2个不饱和脂肪酸成分含量的方法。方法: 采用Ultimate XB-C18色谱柱(4.6 mm×150 mm

5 μm)

流动相乙腈-0.1%磷酸水溶液(88:12)

检测波长203 nm

流速1 mL·min-1

柱温30 ℃。结果: 油酸和亚油酸分别在19.219~ 615.00

3.375~ 108.0 mg·L-1与色谱峰面积呈良好线性关系

加样回收率在81.29%~ 108.44%

RSD在1.3%~ 2.9%;鸦胆子油中的油酸和亚油酸质量分数分别为68.10%和12.49%。结论: 本法无需柱前衍生化处理

具有简单准确

快速高效的特点

为中药鸦胆子油及其制剂的质量控制提供了参考。

Abstract

Objective: A high-performance liquid chromatography-UV method was developed to determine the contents of oleic acid and linoleic acid in Bruceae Fructus oil. Method: A chromatographic process was set up by using column Ultimate XB-C18(4.6 mm×150 mm

5 μm). A mobile phase of 88% acetonitrile-12% phosphoric acid solution (0.1%) was used with flow rate at 1.0 mL·min-1

column temperature at 30 ℃

and determine wavelength at 203 nm. Result: The calibration curve of oleic acid was linear between 19.219-615.00 mg·L-1

and linoleic acid was linear between 3.375-108.0 mg·L-1

with the average recovery 81.29%-108.44% and RSD of 1.3%-2.9%. The contents of oleic acid and linoleic acid in Bruceae Fructus oil were 68.10% and 12.49%. Conclusion: This new analytical method do not need the operation of pre-column derivatization

and with the advantage of simple

accurate and repeatable

which could be a critical reference for quality control of Bruceae Fructus oil pharmaceutical manufacture.

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