Objective: To investigate the effect of modified Renshen Wumei decoction on aquaporins-4(AQP4)and related regulatory factors like potassium sodium adenosine triphosphate enzyme(Na+/K+-ATPase)

protein kinase C(PKC). Method: Fifty SD rats were randomly divided into control group

model group

Baoerkang group

smecta group and modified Renshen Wumei decocotion group. The model was eatablished by multi-factors which includes the cold diarrhea medicine

swimming exhaustive method and hungery. After the modeling

control group and model group were given normal saline ig (20 mL·kg-1)

the rest of the groups were respectively given Renshen Wumei decocotion ig (17.5 g·kg-1)

Smecta ig (4.2 g·kg-1)

Baoerkang ig (3.5 g·kg-1)

once daily for 7 days. qRT-PCR etc techniques and methods were used to detect the gene expression level of colon AQP4

ATPase and PKC. Result: Compared with the control group

the gene expression of AQP4

ATPase and PKC in colon tissues of model rats were significantly lower(P<0.01);compared with the model group

modified Renshen Wumei decoction could significantly improve the gene expression level of AQP4

ATPase and PKC in the colon tissues (P<0.01). Conclusion: Modified Renshen Wumei decocotion can achieve engendering liquid and anti-diarrhea effect through adjusting gene expression levels of AQP4 and related regulatory factors Na+/K+-ATPase

PKC.