Objective: To establish an effictive quality evaluation method of Heracleum candicans roots and determine contents of two active ingredients of bergapten and imperatorin in this medicinal herb. Method: Ten batches of H. candicans roots were collected

HPLC fingerprint was performed in a gradient mode of water-methanol

detection wavelength of 302 nm.Similarity was evaluated with similarity evaluation system for chromatographic fingerprint of tradition Chinese medicine (2004A) issued by state pharmacopoeia committee. Result: HPCL fingerprint similarity among 10 batches of H. candicans roots were more than 0.89 and 11 characteristic common peaks were demarcated.Contents of bergapten and imperatorin in samples were 0.117% and 0.709%. Conclusion: This method is simple and reproducible that can be used for identification and quality evaluation of H. candicans roots.