Objective: To analyze the volatile compounds in Scutellaria barbata from different places. Method: A headspace solid-phase microextraction (HS-SPME) method followed by gas chromatography-mass spectrometry (GC-MS) is described for the analysis of volatile compounds in S. barbata from Jiangsu

Guangxi

Jiangxi

Hunan and Sichuan provinces. The volatile compounds were extracted by PDMS/DVB SPME fiber. The relative content of each component was calculated by the normalization method. Result: There are 45 compounds identified in total and 13 volatile compounds in common from the five places mentioned above. The 13 volatile compounds are 1-octen-3-ol

3

7-dimethyl-1

6-octadien-3-ol methyl ester

phenylethyl alcohol

azulene

2-methoxy-3-(2-propenyl)-phenol

1

2-dimethoxy-4-(2-propenyl)-benzene

pentadecane

propanoic acid

2-methyl-

1-(1

1-dimethylethyl)-2-methyl-1

3-propanediyl ester

hexadecane

6

10

14-trimethyl-2-pentadecanone

hexadecanoic acid methyl ester

dibutyl phthalate

n-hexadecanoic acid. Conclusion: The 13 volatile compounds were identified as the marker components in S. barbata. The results also suggest that there are some differences in the volatile compounds in S.barbata from different places.