Objective: To establish an UPLC method for fingerprints of Artemisae Argyi Folium

and provide reference for quality control of it. Method: The RP-UPLC method was achieved on a uitimate-C18(2.1 mm×100 mm

1.8 μm) column with mobile phase consisting of acetonitrile-0.10% acetic acid water with gradient elution. Flow rate was set at 0.20 mL·min-1. The detection wavelength was set at 290 nm. Then established the fingerprints based on samples from different sources

and the similarity was analyzed. Result: The UPLC fingerprint was established with 36 common peaks. However similarity of the UPLC fingerprints of Artemisae Argyi Folium and non-medicinal parts and adulterants were lower than 0.700 and the common peaks less than 36. Conclusion: A simple

reproducible

and characteristic UPLC fingerprint was developed

which can be used to identify of Artemisae Argyi Folium. The difference of fingerprint indicated that of chemical compositions of Artemisae Argyi Folium

non medicinal parts and adulterants were varied

it should not be mixed.