HPLC测定小儿清肺化痰口服液中5种成分的含量

胡英婕

doi:10.13422/j.cnki.syfjx.2015130062

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HPLC测定小儿清肺化痰口服液中5种成分的含量

化学与分析 | 更新时间：2024-01-04

- HPLC测定小儿清肺化痰口服液中5种成分的含量
- Quantitative Determination of Five Ingredients in Xiaoer Qingfei Huatan Oral Solution by HPLC
- 中国实验方剂学杂志 2015年21卷第13期页码：62-65
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- DOI：10.13422/j.cnki.syfjx.2015130062
  中图分类号： O657.72;R286.0
- 纸质出版日期：2015
- 稿件说明：
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胡英婕. HPLC测定小儿清肺化痰口服液中5种成分的含量[J]. 中国实验方剂学杂志, 2015,21(13):62-65.

HU Ying-jie. Quantitative Determination of Five Ingredients in Xiaoer Qingfei Huatan Oral Solution by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(13): 62-65.
胡英婕. HPLC测定小儿清肺化痰口服液中5种成分的含量[J]. 中国实验方剂学杂志, 2015,21(13):62-65. DOI： 10.13422/j.cnki.syfjx.2015130062.

HU Ying-jie. Quantitative Determination of Five Ingredients in Xiaoer Qingfei Huatan Oral Solution by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(13): 62-65. DOI： 10.13422/j.cnki.syfjx.2015130062.

摘要

目的: 建立HPLC同时测定小儿清肺化痰口服液中5种成分(盐酸麻黄碱、盐酸伪麻黄碱、苦杏仁苷、黄芩苷、白花前胡甲素)含量的方法。方法: 采用ZORBAX SB-C18色谱柱(4.6 mm×250 mm

5 μm)

流动相乙腈-0.1%磷酸水溶液

梯度洗脱

体积流量 0.8 mL·min-1

柱温35 ℃

DAD检测器

检测波长210 nm(盐酸麻黄碱、盐酸伪麻黄碱)

225 nm(苦杏仁苷)

277 nm(黄芩苷)

321 nm(白花前胡甲素)。结果: 5个成分的峰面积与质量浓度的线性关系良好

加样回收率98.52%~99.93%。结论: 该方法简便、准确、灵敏

重复性好

可用于测定小儿清肺化痰口服液中5种成分的含量。

Abstract

Objective: To develop a HPLC method for determining five constituents (ephedrine hydro-chlorid

pseudoephedrine hydrochloride

amygdalim

baicalin and praeruptorin A) in Xiaoer Qingfei Huatan oral solution. Method: Five constituents were analyzed by ZORBAX SB-C18(4.6 mm×250 mm

5 μm) with acetoni-trile-water (containing 0.1% phosphonic acid) as mobile phases in gradient elution

0.8 mL·min-1 as flow-rate and 35 ℃ as temperature of column. The detection wavelengths were at 210 nm for ephedrine hydrochloride and pseudoephedrine hydrochloride

225 nm for amygdalim

277 nm for baicalin and 321 nm for praeruptorin A respectively. Result: Five constituents were seperated perfectly and good linear relationships were obtained. The recoveries were between 98.52%-99.93%%. Conclusion: The developed method is simple

specific

accurate and with good reproducibility

which can be used for quality control of Xiaoer Qingfei Huatan oral solution.

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